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AB192543

Anti-GPI-PLD antibody

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(2 Publications)

Rabbit Polyclonal GPI-PLD antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GPLD1 aa 300 to C-terminus.

View Alternative Names

PIGPLD1, GPLD1, Phosphatidylinositol-glycan-specific phospholipase D, PI-G PLD, Glycoprotein phospholipase D, Glycosyl-phosphatidylinositol-specific phospholipase D, GPI-PLD, GPI-specific phospholipase D

1 Images
Western blot - Anti-GPI-PLD antibody (AB192543)
  • WB

Supplier Data

Western blot - Anti-GPI-PLD antibody (AB192543)

All lanes:

Western blot - Anti-GPI-PLD antibody (ab192543)

Lane 1:

A549 whole cell lysate

Lane 2:

Mouse brain tissue lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GPLD1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P80108

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab192543 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycosylphosphatidylinositol specific phospholipase D1 (GPI-PLD) is an enzyme that hydrolyzes glycosylphosphatidylinositol (GPI)-anchored proteins. Also known as GPLD1 it acts by cleaving the inositol phosphate linkage. GPI-PLD possesses a molecular mass of about 110 kDa and is found extensively in the plasma signaling its secretion from the liver. In certain studies researchers occasionally refer to it by its involved function in detaching GPI proteins.
Biological function summary

GPI-PLD participates in remodeling the cell surface by releasing certain GPI-anchored proteins. It impacts cellular events like signal transduction and cell adhesion. GPI-PLD functions independently not as part of a larger complex. Observation of GPI-PLD activities reveals involvement in regulating immune responses as well as interactions with other proteins on cell membranes. Its ability to influence GPI protein release affects how cells communicate and adapt in various biological contexts.

Pathways

The enzyme GPI-PLD plays a role in pathways involving lipid metabolism and the immune system particularly complement system activation. GPI-PLD collaborates with proteins such as phospholipase C in lipid regulatory pathways. Its activity impacts the balance and turnover of lipids which affects the complement cascade by modulating membrane-bound proteins. Through these interactions GPI-PLD participates in refining how immune responses unfold affecting related biological processes.

Research shows that GPI-PLD has connections with conditions like atherosclerosis and diabetes. Changes in its enzymatic activity and expression levels impact lipid metabolism which ties to atherosclerosis development. In diabetes improper regulation of GPI-PLD can alter inflammatory and immune responses. The enzyme's activity further connects with lipoprotein interactions placing emphasis on its link with apolipoprotein AI indicating a broader influence in disorders related to lipid metabolism and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the inositol phosphate linkage of glycosylphosphatidylinositol (GPI)-anchored membrane proteins, thereby releasing them from the cell surface. Cleaves on the inositol side of the anchor, leaving the phosphate group attached to the membrane (PubMed : 17720976, PubMed : 2760042). This release of GPI-anchored proteins can modulate diverse biological processes, including the activation of signaling pathways (PubMed : 17720976).
See full target information GPLD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 17:3725-3739 PubMed33783314

2021

The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Chun Chang,Ran N Tao,Chong Teik Tan,Ya Jun Wu,Boon Huat Bay,Victor C Yu

JCI insight 5: PubMed31830004

2020

Structure-function relationships of HDL in diabetes and coronary heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Cardner,Mustafa Yalcinkaya,Sandra Goetze,Edlira Luca,Miroslav Balaz,Monika Hunjadi,Johannes Hartung,Andrej Shemet,Nicolle Kränkel,Silvija Radosavljevic,Michaela Keel,Alaa Othman,Gergely Karsai,Thorsten Hornemann,Manfred Claassen,Gerhard Liebisch,Erick Carreira,Andreas Ritsch,Ulf Landmesser,Jan Krützfeldt,Christian Wolfrum,Bernd Wollscheid,Niko Beerenwinkel,Lucia Rohrer,Arnold von Eckardstein
View all publications
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Product promise

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