AB224728

Anti-Gpihbp1 antibody

Name: Anti-Gpihbp1 antibody (ab224728) | Abcam
Brand: Abcam Limited
SKU: AB224728

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(3 Publications)

Rabbit Polyclonal Gpihbp1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GPIHBP1.

View Alternative Names

HBP1, GPIHBP1, Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1, GPI-HBP1, GPI-anchored HDL-binding protein 1, High density lipoprotein-binding protein 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gpihbp1 antibody (AB224728)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gpihbp1 antibody (AB224728)

Paraffin-embedded human skin tissue stained for Gpihbp1 with ab224728 at 1/20 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Gpihbp1 antibody (AB224728)

All lanes:

Western blot - Anti-Gpihbp1 antibody (ab224728) at 1/500 dilution

Lane 1:

Rat liver tissue lysate

Lane 2:

Mouse heart tissue lysate

Lane 3:

Mouse lung tissue lysate

Lane 4:

Mouse kidney tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GPIHBP1.

Q8IV16

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gpihbp1 also known as GPIHBP1 is a glycosylphosphatidylinositol-anchored protein that plays a mechanical role in transporting lipoprotein lipase (LPL) to the capillary lumen. This protein has a molecular weight of about 20 kDa. GPIHBP1 is expressed mainly in the endothelial cells of capillaries within adipose tissue heart and skeletal muscle. It binds with LPL and anchors it to the endothelial cell surface facilitating the hydrolysis of triglycerides in circulating chylomicrons and very-low-density lipoproteins.

Biological function summary

The presence of GPIHBP1 is essential for the proper functioning of the lipolysis process enabling the breakdown of triglycerides into free fatty acids and glycerol. It forms a stable complex with LPL that is necessary for LPL's movement from interstitial spaces to the luminal surface of capillaries. Without this function the processing of lipid particles in the bloodstream would be disrupted affecting the availability of free fatty acids for uptake by tissues.

Pathways

GPIHBP1 participates significantly in the lipid metabolism pathway specifically concerning the regulation of plasma triglyceride levels. This pathway includes key interactions with other proteins such as chylomicrons which it assists in metabolizing and apolipoprotein C-II a cofactor required for LPL activity. GPIHBP1 ensures that LPL is correctly localized to execute its function in hydrolyzing triglycerides in these pathways.

Dysfunction or deficiency of GPIHBP1 can result in severe hypertriglyceridemia a condition linked to an increased risk of pancreatitis. Mutations affecting GPIHBP1 can impair LPL function leading to the accumulation of triglyceride-rich lipoproteins in the bloodstream which can potentially cause acute pancreatitis. The protein also indirectly connects to Apolipoprotein C-II through its role in LPL regulation as both are critical for lipid metabolism and energy homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the transport of lipoprotein lipase LPL from the basolateral to the apical surface of endothelial cells in capillaries (By similarity). Anchors LPL on the surface of endothelial cells in the lumen of blood capillaries (By similarity). Protects LPL against loss of activity, and against ANGPTL4-mediated unfolding (PubMed : 27929370, PubMed : 29899144). Thereby, plays an important role in lipolytic processing of chylomicrons by LPL, triglyceride metabolism and lipid homeostasis (PubMed : 19304573, PubMed : 21314738). Binds chylomicrons and phospholipid particles that contain APOA5 (PubMed : 17997385, PubMed : 19304573). Binds high-density lipoprotein (HDL) and plays a role in the uptake of lipids from HDL (By similarity).

See full target information GPIHBP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 14:491-512 PubMed40114952

2025

Aspirin impedes non-small cell lung cancer development via fine-tuning the CD36 localization regulated by GPIHBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Dujuan Qiao,Jia Chen,Ya Gao,Katsuhiro Okuda,Yoshihisa Shimada,Linong Yao

Translational cancer research 13:2691-2703 PubMed38988918

2024

Increase in GPIHBP1 expression in advanced stage colorectal cancer indicates poor immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Min Gao,Liting Liao,Zhijie Lin,Xiangyu Hu,Linhui Jia,Weijuan Gong,Xiaoqin Jia

Cancers 13: PubMed33669398

2021

COMMD1, from the Repair of DNA Double Strand Breaks, to a Novel Anti-Cancer Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Amila Suraweera,Pascal H G Duijf,Christian Jekimovs,Karsten Schrobback,Cheng Liu,Mark N Adams,Kenneth J O'Byrne,Derek J Richard

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Gpihbp1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gpihbp1 antibody (AB224728)

Western blot - Anti-Gpihbp1 antibody (AB224728)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise