Rabbit Polyclonal Gpihbp1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GPIHBP1.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GPIHBP1. Database link Q8IV16
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Mediates the transport of lipoprotein lipase LPL from the basolateral to the apical surface of endothelial cells in capillaries (By similarity). Anchors LPL on the surface of endothelial cells in the lumen of blood capillaries (By similarity). Protects LPL against loss of activity, and against ANGPTL4-mediated unfolding (PubMed:27929370, PubMed:29899144). Thereby, plays an important role in lipolytic processing of chylomicrons by LPL, triglyceride metabolism and lipid homeostasis (PubMed:19304573, PubMed:21314738). Binds chylomicrons and phospholipid particles that contain APOA5 (PubMed:17997385, PubMed:19304573). Binds high-density lipoprotein (HDL) and plays a role in the uptake of lipids from HDL (By similarity).
Alternative names
HBP1, GPIHBP1, Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1, GPI-HBP1, GPI-anchored HDL-binding protein 1, High density lipoprotein-binding protein 1
Recommended products
Rabbit Polyclonal Gpihbp1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GPIHBP1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GPIHBP1. Database link Q8IV16
- Purification technique
Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gpihbp1 also known as GPIHBP1 is a glycosylphosphatidylinositol-anchored protein that plays a mechanical role in transporting lipoprotein lipase (LPL) to the capillary lumen. This protein has a molecular weight of about 20 kDa. GPIHBP1 is expressed mainly in the endothelial cells of capillaries within adipose tissue heart and skeletal muscle. It binds with LPL and anchors it to the endothelial cell surface facilitating the hydrolysis of triglycerides in circulating chylomicrons and very-low-density lipoproteins.
- Biological function summary
The presence of GPIHBP1 is essential for the proper functioning of the lipolysis process enabling the breakdown of triglycerides into free fatty acids and glycerol. It forms a stable complex with LPL that is necessary for LPL's movement from interstitial spaces to the luminal surface of capillaries. Without this function the processing of lipid particles in the bloodstream would be disrupted affecting the availability of free fatty acids for uptake by tissues.
- Pathways
GPIHBP1 participates significantly in the lipid metabolism pathway specifically concerning the regulation of plasma triglyceride levels. This pathway includes key interactions with other proteins such as chylomicrons which it assists in metabolizing and apolipoprotein C-II a cofactor required for LPL activity. GPIHBP1 ensures that LPL is correctly localized to execute its function in hydrolyzing triglycerides in these pathways.
- Associated diseases and disorders
Dysfunction or deficiency of GPIHBP1 can result in severe hypertriglyceridemia a condition linked to an increased risk of pancreatitis. Mutations affecting GPIHBP1 can impair LPL function leading to the accumulation of triglyceride-rich lipoproteins in the bloodstream which can potentially cause acute pancreatitis. The protein also indirectly connects to Apolipoprotein C-II through its role in LPL regulation as both are critical for lipid metabolism and energy homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Gpihbp1 antibody (ab224728)
All lanes: Western blot - Anti-Gpihbp1 antibody (ab224728) at 1/500 dilution
Lane 1: Rat liver tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse kidney tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gpihbp1 antibody (ab224728)
Paraffin-embedded human skin tissue stained for Gpihbp1 with ab224728 at 1/20 dilution in immunohistochemical analysis.
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