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AB214459

Anti-GPM6B antibody

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(1 Publication)

Rabbit Polyclonal GPM6B antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GPM6B aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

M6B, GPM6B, Neuronal membrane glycoprotein M6-b, M6b

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPM6B antibody (AB214459)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPM6B antibody (AB214459)

Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat brain tissue labeling GPM6B with ab214459 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-GPM6B antibody (AB214459)
  • WB

Supplier Data

Western blot - Anti-GPM6B antibody (AB214459)

All lanes:

Western blot - Anti-GPM6B antibody (ab214459) at 1/300 dilution

All lanes:

Mouse brain lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GPM6B aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13491

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Proteins & Peptides

AB132899

Recombinant Human GPM6B protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glycoprotein M6B commonly known as GPM6B is a membrane glycoprotein that belongs to the proteolipid protein family. This protein has a molecular mass of approximately 33 kilodaltons. GPM6B is expressed in various tissues with a strong presence in the brain particularly in neurons and glial cells. It is also found in bone and platelets indicating its diverse roles in different physiological contexts.
Biological function summary

The glycoprotein M6B contributes to several cellular processes such as neurite outgrowth and synapse assembly. It is known to participate in myelination and cell adhesion activities. Researchers have identified GPM6B to be part of a membrane microdomain where it may work with other proteins to mediate these cellular interactions. Its role in bone homeostasis further highlights its biological significance across distinct cellular environments.

Pathways

Glycoprotein M6B is significantly involved in the calcium signaling pathway and the integrin signaling pathway. Within these pathways GPM6B works in conjunction with integrins and calcium channels influencing cellular responses to mechanical stimuli and cell-extracellular matrix adhesion. These pathways highlight GPM6B's role in maintaining cellular structure and signaling transduction as they contribute to broader cellular functionalities.

Abnormal expression or mutations of glycoprotein M6B have been linked to neurological disorders like autism spectrum disorder and the bone disease osteoporosis. The involvement of GPM6B in these conditions may also relate to its interaction with proteins such as integrins. These interactions can affect cellular procedures critically important for maintaining neural network connectivity and bone density revealing therapeutic research potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake.
See full target information GPM6B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuropathology and experimental neurology 78:819-843 PubMed31400116

2019

Anti-Myelin Proteolipid Protein Peptide Monoclonal Antibodies Recognize Cell Surface Proteins on Developing Neurons and Inhibit Their Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Raymond A Sobel,Mary Jane Eaton,Prajakta Dilip Jaju,Eugene Lowry,Julian R Hinojoza
View all publications
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Product promise

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