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Rabbit Recombinant Monoclonal GPNMB antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.


Images

Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] (AB188222), expandable thumbnail
  • Western blot - Anti-GPNMB antibody [EPR18226-147] (AB188222), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] (AB188222), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] (AB188222), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Mouse
Tested
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Could be a melanogenic enzyme.

Alternative names

Dchil, Hgfin, Nmb, Gpnmb, Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin

Recommended products

Rabbit Recombinant Monoclonal GPNMB antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18226-147
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.

Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

Associated diseases and disorders

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] (ab188222), expandable thumbnail

    Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] (ab188222)

    GPNMB was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab188222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: L-929 whole cell lysate 10 μg (Input).

    Lane 2: ab188222 IP in L-929 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab188222 in L-929 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 minute.

    All lanes: Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] (ab188222)

    Developed using the ECL technique.

    Predicted band size: 64 kDa

    Observed band size: 65 kDa, 95 kDa

  • Western blot - Anti-GPNMB antibody [EPR18226-147] (ab188222), expandable thumbnail

    Western blot - Anti-GPNMB antibody [EPR18226-147] (ab188222)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    Negative control: EL4 (PMID: 19320736).

    All lanes: Western blot - Anti-GPNMB antibody [EPR18226-147] (ab188222) at 1/5000 dilution

    Lane 1: L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 20 µg

    Lane 2: B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate at 20 µg

    Lane 3: EL4 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa

    Observed band size: 120 kDa, 65 kDa, 95 kDa

    Exposure time: 15s

  • Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] (ab188222), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] (ab188222)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4 (mouse lymphoma T lymphocyte cell line; left panel) and L-929 (mouse connective tissue fibroblast cell line; right panel) cell lines labeling GPNMB with ab188222 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: EL4 (PMID: 19320736) (left panel).

  • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] (ab188222), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] (ab188222)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L-929 (mouse connective tissue fibroblast cell line) and EL4 (mouse lymphoma T lymphocyte cell line) cells labeling GPNMB with ab188222 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining in L-929 cells.

    Negative control: EL4 (PMID:19320736).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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Product protocols

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