Rabbit Recombinant Monoclonal GPNMB antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (AB234529), expandable thumbnail](https://content.abcam.com/products/images/gpnmb-antibody-epr18226-147-bsa-and-azide-free-ab234529--immunoprecipitation-img9112.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (AB234529)")
![Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (AB234529), expandable thumbnail](https://content.abcam.com/products/images/gpnmb-antibody-epr18226-147-bsa-and-azide-free-ab234529--flow-cytometry-intracellular-img13171.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (AB234529)")
![Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (AB234529), expandable thumbnail](https://content.abcam.com/products/images/gpnmb-antibody-epr18226-147-bsa-and-azide-free-ab234529--immunocytochemistry-immunofluorescence-img38523.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (AB234529)")
Publications
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- Nature biotechnology 40:1360-13692022DestVI identifies continuums of cell types in spatial transcriptomics data.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomain Lopez,Baoguo Li,Hadas Keren-Shaul,Pierre Boyeau,Merav Kedmi,David Pilzer,Adam Jelinski,Ido Yofe,Eyal David,Allon Wagner,Can Ergen,Yoseph Addadi,Ofra Golani,Franca Ronchese,Michael I Jordan,Ido Amit,Nir YosefPubMed 35449415
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Mouse | Tested | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Alternative names
Dchil, Hgfin, Nmb, Gpnmb, Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin
Recommended products
Rabbit Recombinant Monoclonal GPNMB antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18226-147
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab234529 is the carrier-free version of Anti-GPNMB antibody [EPR18226-147] ab188222.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.
- Biological function summary
GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.
- Pathways
GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.
- Associated diseases and disorders
GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529), expandable thumbnail](https://content.abcam.com/products/images/gpnmb-antibody-epr18226-147-bsa-and-azide-free-ab234529--immunoprecipitation-img9112.jpg "Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529)")
Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529)
GPNMB was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with Anti-GPNMB antibody [EPR18226-147] ab188222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-GPNMB antibody [EPR18226-147] ab188222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: L-929 whole cell lysate 10 μg (Input).
Lane 2: Anti-GPNMB antibody [EPR18226-147] ab188222 IP in L-929 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GPNMB antibody [EPR18226-147] ab188222 in L-929 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GPNMB antibody [EPR18226-147] ab188222).
All lanes: Immunoprecipitation - Anti-GPNMB antibody [EPR18226-147] (Anti-GPNMB antibody [EPR18226-147] ab188222)
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 65 kDa, 95 kDa
![Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529), expandable thumbnail](https://content.abcam.com/products/images/gpnmb-antibody-epr18226-147-bsa-and-azide-free-ab234529--flow-cytometry-intracellular-img13171.jpg "Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529)")
Flow Cytometry (Intracellular) - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4 (mouse lymphoma T lymphocyte cell line; left panel) and L-929 (mouse connective tissue fibroblast cell line; right panel) cell lines labeling GPNMB with Anti-GPNMB antibody [EPR18226-147] ab188222 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: EL4 (PMID: 19320736) (left panel).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GPNMB antibody [EPR18226-147] ab188222).
![Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529), expandable thumbnail](https://content.abcam.com/products/images/gpnmb-antibody-epr18226-147-bsa-and-azide-free-ab234529--immunocytochemistry-immunofluorescence-img38523.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529)")
Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR18226-147] - BSA and Azide free (ab234529)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L-929 (mouse connective tissue fibroblast cell line) and EL4 (mouse lymphoma T lymphocyte cell line) cells labeling GPNMB with Anti-GPNMB antibody [EPR18226-147] ab188222 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in L-929 cells.
Negative control: EL4 (PMID:19320736).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GPNMB antibody [EPR18226-147] ab188222).
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