AB272357

Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GPNMB antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Dchil, Hgfin, Nmb, Gpnmb, Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin

1 Images

Sandwich ELISA - Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector) (AB272357)

sELISA

Supplier Data

Sandwich ELISA - Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector) (AB272357)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270892).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22150-10

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab272357 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse GPNMB. The recommended pair for sandwich ELISA is:
Capture: ab272356, Mouse GPNMB Capture Antibody (unconjugated)
Detector: ab272357, Mouse GPNMB Detector Antibody (unconjugated)
The reference range value is 0.78125 - 50 ng/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.

Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could be a melanogenic enzyme.

See full target information Gpnmb

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector) (AB272357)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise