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Rabbit Recombinant Monoclonal GPNMB antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (AB317326), expandable thumbnail
  • Western blot - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (AB317326), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (AB317326), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (AB317326), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (AB317326), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)IP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Could be a melanogenic enzyme.

Alternative names

Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin, Nmb, Gpnmb, Dchil, Hgfin

Recommended products

Rabbit Recombinant Monoclonal GPNMB antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat samples.

Alternative names

Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin, Nmb, Gpnmb, Dchil, Hgfin

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR29063-10

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab317326 is the carrier-free version of Anti-GPNMB antibody [EPR29063-10] ab317325.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.

Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

Associated diseases and disorders

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Western blot - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Low expression: testis

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPNMB antibody [EPR29063-10] (Anti-GPNMB antibody [EPR29063-10] ab317325) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Lane 3: Rat testis tissue lysate at 20 µg

    Lane 4: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 65 kDa, 120 kDa, 36 kDa

    Exposure time: 180s

    Low expression: testis

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Western blot - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Negative control: EL4(PMID: 19320736), 4T1(PMID: 20711474)

    The identity of the lower MW band at approximately 15 kDa (in lane1 and 2) is unknown.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPNMB antibody [EPR29063-10] (Anti-GPNMB antibody [EPR29063-10] ab317325) at 1/1000 dilution

    Lane 1: B16-F10 (Mouse skin melanoma) whole cell lysate at 20 µg

    Lane 2: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate at 20 µg

    Lane 3: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 4: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 95 kDa, 120 kDa, 36 kDa

    Exposure time: 37s

    Negative control: EL4(PMID: 19320736), 4T1(PMID: 20711474)

    The identity of the lower MW band at approximately 15 kDa (in lane1 and 2) is unknown.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized B16-F0 (mouse melanoma epithelial-like cell) cells labelling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/500 (1.036 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in B16-F0 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: EL4 (PMID: 19320736).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded A) B16-F0 (mouse melanoma epithelial-like cell) cell pellet; B) EL4 (mouse lymphoma T lymphocyte) cell pellet tissue labeling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on A) B16-F0 cell pellet; no staining on B) EL4 cell pellet.

    The section was incubated with Anti-GPNMB antibody [EPR29063-10] ab317325 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: scattered staining on immune cells of rat testis.

    The section was incubated with Anti-GPNMB antibody [EPR29063-10] ab317325 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: scattered staining on immune cells of mouse testis.

    The section was incubated with Anti-GPNMB antibody [EPR29063-10] ab317325 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat spleen.

    The section was incubated with Anti-GPNMB antibody [EPR29063-10] ab317325 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung cancer (PMID: 29857071).

    The section was incubated with Anti-GPNMB antibody [EPR29063-10] ab317325 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [EPR29063-10] - BSA and Azide free (ab317326)

    This data was developed using Anti-GPNMB antibody [EPR29063-10] ab317325, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GPNMB with Anti-GPNMB antibody [EPR29063-10] ab317325 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen.

    The section was incubated with Anti-GPNMB antibody [EPR29063-10] ab317325 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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