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Rabbit Recombinant Monoclonal GPNMB antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

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Images

Flow Cytometry - Anti-GPNMB antibody [SP299] (AB227695), expandable thumbnail
  • Western blot - Anti-GPNMB antibody [SP299] (AB227695), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (AB227695), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (AB227695), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (AB227695), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Primary incubation for 30 minutes at 4°C.

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

Primary incubation for 1 hour at room temperature.

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Could be a melanogenic enzyme.

Alternative names

Transmembrane glycoprotein NMB, Hematopoietic growth factor inducible neurokinin-1 type, HGFIN, NMB, UNQ1725/PRO9925, GPNMB

Recommended products

Rabbit Recombinant Monoclonal GPNMB antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

Alternative names

Transmembrane glycoprotein NMB, Hematopoietic growth factor inducible neurokinin-1 type, HGFIN, NMB, UNQ1725/PRO9925, GPNMB

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP299

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.

Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

Associated diseases and disorders

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Flow Cytometry - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Flow Cytometry - Anti-GPNMB antibody [SP299] (ab227695)

    Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells, labeling GPNMB with ab227695 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Western blot - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Western blot - Anti-GPNMB antibody [SP299] (ab227695)

    All lanes: Western blot - Anti-GPNMB antibody [SP299] (ab227695) at 1/100 dilution

    All lanes: A-375 (human malignant melanoma cell line) whole cell lysate

    Predicted band size: 64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human lung tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human colon tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPNMB antibody [SP299] (ab227695)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Western blot - Anti-GPNMB antibody [SP299] (ab227695)

    ab227695 was shown to react with GPNMB in wild-type U-87 MG cells in Western blot with loss of signal observed in a GPNMB siRNA knockdown cell line. Cell lysates from wild-type U-87 MG transfected with either scrambled siRNA or GPNMB siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab227695 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-GPNMB antibody [SP299] (ab227695) at 1/500 dilution

    Lane 1: Wild-type U-87 MG transfected with scrambled siRNA control lysate at 60 µg

    Lane 2: U-87 MG transfected with siRNA specifically targeting GPNMB cell lysate at 60 µg

  • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [SP299] (ab227695), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [SP299] (ab227695)

    ab227695 was shown to react with GPNMB in wild-type U-87 MG cells in immunocytochemistry with loss of signal observed in a GPNMB siRNA knockdown cell line. Wild-type and siRNA knockdown cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with ab227695 at 1/500 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 ?g/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (siRNA knockdown) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and siRNA knockdown are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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