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AB188637

Anti-GPR133 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal GPR133 antibody. N-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ADGRD1.

View Alternative Names

GPR133, PGR25, ADGRD1, Adhesion G-protein coupled receptor D1, G-protein coupled receptor 133, G-protein coupled receptor PGR25

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR133 antibody - N-terminal (AB188637)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR133 antibody - N-terminal (AB188637)

Immunohistochemical analysis of formalin/PFA fixed paraffin embedded Human ovary epithelium tissue sections labeling GPR133 using ab188637 at 30 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ADGRD1. The exact immunogen used to generate this antibody is proprietary information.

Q6QNK2

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except ENTPD4 (50%).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15-30 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR133 also known as ADGRD1 is a member of the adhesion G protein-coupled receptor (GPCR) family. It possesses a large extracellular domain and a characteristic seven-transmembrane domain typical of GPCRs with a molecular mass approximately 100 kDa. GPR133 expresses broadly in the central nervous system and several peripheral tissues including the lung and heart. This pattern of expression suggests its involvement in various physiological functions.
Biological function summary

Adhesion G protein-coupled receptor GPR133 has roles in cellular signaling and communication. It participates in the regulation of processes such as cell adhesion and migration. GPR133 does not act alone; it associates with other proteins to affect physiological functions though it is not strictly part of a larger protein complex. Its broad expression in several tissues indicates its functional versatility.

Pathways

Research links GPR133 to significant signaling pathways like the Wnt signaling pathway and the G-protein signaling pathway. It interacts with the proteins involved in these pathways to modulate their downstream effects impacting cellular processes such as proliferation and differentiation. Partner proteins such as heterotrimeric G proteins directly associate with GPR133 playing roles in the signal transduction processes initiated by this receptor.

GPR133 associates with conditions such as glioblastoma and asthma. It often operates alongside other proteins involved in these conditions such as EGFR in glioblastoma and the IL-4 receptor in asthma. Alterations in the expression or function of GPR133 may contribute to the pathogenesis of these diseases by affecting cell signaling pathways presenting it as a potential therapeutic target for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adhesion G-protein coupled receptor (aGPCR) for androgen hormone 5alpha-dihydrotestosterone (5alpha-DHT), also named 17beta-hydroxy-5alpha-androstan-3-one, the most potent hormone among androgens (PubMed : 39884271). Also activated by methenolone drug (PubMed : 39884271). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 39884271). ADGRD1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed : 22025619, PubMed : 22575658, PubMed : 35447113, PubMed : 39884271). Acts as a 5alpha-DHT receptor in muscle cells, thereby increasing intracellular cyclic AMP (cAMP) levels and enhancing muscle strength (PubMed : 39884271).
See full target information ADGRD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 184:3056-3074.e21 PubMed33932339

2021

A cellular and spatial map of the choroid plexus across brain ventricles and ages.

Applications

Unspecified application

Species

Unspecified reactive species

Neil Dani,Rebecca H Herbst,Cristin McCabe,Gilad S Green,Karol Kaiser,Joshua P Head,Jin Cui,Frederick B Shipley,Ahram Jang,Danielle Dionne,Lan Nguyen,Christopher Rodman,Samantha J Riesenfeld,Jan Prochazka,Michaela Prochazkova,Radislav Sedlacek,Feng Zhang,Vitezslav Bryja,Orit Rozenblatt-Rosen,Naomi Habib,Aviv Regev,Maria K Lehtinen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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