Rabbit Polyclonal GPR158 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GPR158 aa 900-1100.
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- Cell death & disease 13:2722022Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBao-Dong Wang,Xiao-Jun Yu,Ji-Chun Hou,Bo Fu,Hao Zheng,Qi-Kun Liu,Shan-Xi Wang,Zheng-Gang Bi,Yang CaoPubMed 35347106
- Molecular & cellular proteomics : MCP 18:1669-16822019Mapping Spatiotemporal Microproteomics Landscape in Experimental Model of Traumatic Brain Injury Unveils a link to Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhalil Mallah et. al.PubMed 31204315
- Oncogene 37:4313-43332018Inhibition of GPR158 by microRNA-449a suppresses neural lineage of glioma stem/progenitor cells and correlates with higher glioma grades.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNingning Li et. al.PubMed 29720725
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GPR158 aa 900-1100. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T848
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Metabotropic receptor for glycine that controls synapse formation and function in the brain (PubMed:36996198). Acts as an atypical G-protein coupled receptor that recruits and regulates the RGS7-GNB5 complex instead of activating G proteins (PubMed:31189666, PubMed:36996198). In absence of glycine ligand, promotes the GTPase activator activity of RGS7, increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:36996198). Glycine-binding changes the conformation of the intracellular surface, inhibiting the GTPase activator activity of the RGS7-GNB5 complex, promoting G protein alpha subunits into their active GTP-bound form and regulating cAMP levels (PubMed:36996198). Also able to bind taurine, a compound closely related to glycine, but with a two-fold lower affinity (PubMed:36996198). Glycine receptor-dependent regulation of cAMP controls key ion channels, kinases and neurotrophic factors involved in neuronal excitability and synaptic transmission (PubMed:36996198). Plays a pivotal role in regulating mood and cognition via its ability to regulate neuronal excitability in L2/L3 pyramidal neurons of the prefrontal cortex (By similarity). Also involved in spatial learning by regulating hippocampal CA1 neuronal excitability (By similarity). Acts as a synaptic organizer in the hippocampus, required for proper mossy fiber-CA3 neurocircuitry establishment, structure and function: induces presynaptic differentiation in contacting axons via its interaction with GPC4 (By similarity). In addition to glycine, may also act as a receptor for osteocalcin (BGLAP) hormone: osteocalcin-binding initiates a signaling response that prevents neuronal apoptosis in the hippocampus and regulates the synthesis of neurotransmitters (By similarity).
Alternative names
KIAA1136, GPR158, Metabotropic glycine receptor, mGlyR, G-protein coupled receptor 158
Recommended products
Rabbit Polyclonal GPR158 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GPR158 aa 900-1100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GPR158 aa 900-1100. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T848
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR158 also known as G protein-coupled receptor 158 is an orphan receptor with a molecular mass of approximately 92 kDa. This protein is part of the GPCR family which has a structure characterized by seven transmembrane domains. GPR158 is highly expressed in the brain notably in regions such as the prefrontal cortex and hippocampus which have roles in cognitive and emotional processing. It is also present in other tissues but at lower levels indicating a more specialized function in the central nervous system.
- Biological function summary
GPR158 interacts with certain signaling molecules and is involved in modulating neurotransmission. It does not operate alone; it can form complexes with other proteins like RGS proteins which help regulate GPCR signaling. This interaction influences cellular responses to various stimuli. GPR158 plays a role in neurons and is involved in pathways that control synaptic plasticity and neuronal survival which are essential for learning and memory processes.
- Pathways
Research indicates that GPR158 is part of both the PI3K-Akt signaling and the G protein signaling pathways. These pathways are critical for cell growth survival and metabolism. GPR158 connects with proteins such as Akt an important player in mediating several GPCR functions. Through its involvement in these pathways GPR158 influences cellular responses to growth factors and stress linking external signals to internal cellular mechanisms.
- Associated diseases and disorders
GPR158 shows relevance to mental health conditions and certain cancers. In neuropsychiatric disorders like depression altered GPR158 expression levels affect mood regulation potentially through its interactions with neurotransmitter systems. Additionally in prostate cancer GPR158 connects to the androgen receptor pathway hinting at its role in tumor progression. Understanding these connections can help in developing therapeutic strategies targeting GPR158-related pathways in disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR158 antibody (ab121388)
ab121388 at 1/100 dilution staining GPR158 in Paraffin-emebedded Human gall bladder tissue by Immunohistochemistry showing weak nuclear positivity in glandular cells.
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