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AB189282

Anti-GPR171 antibody

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Rabbit Polyclonal GPR171 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GPR171 aa 100-200.

View Alternative Names

H963, GPR171, G-protein coupled receptor 171, G-protein coupled receptor H963

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR171 antibody (AB189282)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR171 antibody (AB189282)

Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate tissue labeling GPR171 with ab189282 at 5 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-GPR171 antibody (AB189282)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPR171 antibody (AB189282)

Immunofluorescent analysis of MCF7 cells labeling GPR171 with ab189282 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.

Western blot - Anti-GPR171 antibody (AB189282)
  • WB

Supplier Data

Western blot - Anti-GPR171 antibody (AB189282)

All lanes:

Western blot - Anti-GPR171 antibody (ab189282) at 1/500 dilution

Lane 1:

K562 cell extract with Without synthesized peptide.

Lane 2:

K562 cell extract with With synthesized peptide.

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GPR171 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

O14626

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR171 also known as Probable G-protein coupled receptor 171 is a type of receptor involved in signal transduction. With a mass of approximately 40kDa GPR171 is part of the GPCR family. It is expressed in various tissues including the brain and immune cells. This receptor functions by interacting with ligands and activating intracellular signaling cascades which are integral to the receptor’s mechanical role in physiological processes.
Biological function summary

GPR171 affects neurotransmission and immune response regulation. It interacts with other proteins to form complexes that modulate cellular communication. Its expression within the brain indicates a potential role in neurological functions while in immune cells GPR171 participates in modulating immune responses. The wide expression pattern suggests that it may have multiple roles across different cell types.

Pathways

The GPR171 mechanism is associated with neuropeptide signaling and inflammatory response pathways. It interacts with other GPCRs and intracellular signaling proteins which are essential for transmitting signals that trigger these pathways. GPR171 may also relate to proteins like opioid receptors involved in modifying pain perception and pleasure sensations highlighting its potential influence on the nervous system.

GPR171 has connections to neurological and inflammatory conditions. Its role in brain signaling pathways links it to potential implications in disorders such as anxiety where it may interact with other GPCR proteins. In inflammatory disorders GPR171 may influence immune cell activity possibly relating to proteins like cytokine receptors known to mediate immune responses. Understanding these connections helps to provide insight into therapeutic targeting of GPR171 in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for Big LEN, a 16-amino acid neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Acts through a G(i)-alpha-mediated pathway in response to Big LEN. Big LEN-GPR171 system plays an important role in regulating feeding and metabolism. Also plays a role in modulating fear and anxiety-like behaviors in the basolateral amygdala. Big LEN-GPR171 modulates the mu-type opioid receptor signaling and antinociception (By similarity). Acts as a negative regulator T cell function (PubMed : 34615877).
See full target information GPR171
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Product promise

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