AB184558

Anti-GPR176 antibody [EPR13983] - N-terminal

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GPR176 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

G-protein coupled receptor 176, HB-954, GPR176

1 Images

Supplier Data

Western blot - Anti-GPR176 antibody [EPR13983] - N-terminal (AB184558)

All lanes:

Western blot - Anti-GPR176 antibody [EPR13983] - N-terminal (ab184558) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

BxPC3 cell line lysate at 20 µg

Lane 3:

MCF7 cell line lysate at 20 µg

Lane 4:

SH SY5Y cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13983

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR176 also known as G protein-coupled receptor 176 is a member of the G protein-coupled receptor family. The receptor has a molecular mass of around 47 kDa. Usually you find it expressed in various tissues including the brain and lymphoid organs. As a GPCR GPR176 may act through activating specific G proteins that transmit signals inside cells leading to various cellular responses.

Biological function summary

GPR176 shows roles in regulating circadian rhythms and immune system functions. It appears not to form a complex but influences biological processes through its signaling pathways. Evidence suggests that it modulates certain aspects of the immune response by impacting the behavior of immune cells.

Pathways

GPR176 participates in pathways connected to the regulation of circadian rhythms and immune system signaling. It interacts with pathways involving other GPCRs which are critical for maintaining physiological homeostasis. Proteins such as CLOCK and PER1 in the circadian rhythm pathway are related to the actions of GPR176 helping coordinate daily physiological cycles.

Researchers link GPR176 to conditions like immune dysfunction and mood disorders. In particular dysregulation of circadian rhythms can have ties with mood disorders where GPR176 plays a role. The target also shows relevance to immune-related conditions interconnected with proteins like NF-kB which influence inflammation and immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in normal circadian rhythm behavior. Acts through the G-protein subclass G(z)-alpha and has an agonist-independent basal activity to repress cAMP production.

See full target information GPR176

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39941126

2025

Gender Differences in Adenine Diet-Induced Kidney Toxicity: The Impact of 17β-Estradiol on Renal Inflammation and Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sugyeong Ha,Minjung Son,Jeongwon Kim,Doyeon Kim,Mi-Jeong Kim,Jian Yoo,Byeong Moo Kim,Donghwan Kim,Hae Young Chung,Ki Wung Chung

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPR176 antibody [EPR13983] - N-terminal

Western blot - Anti-GPR176 antibody [EPR13983] - N-terminal (AB184558)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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