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Rabbit Recombinant Monoclonal GPR177/WLS antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (AB323884), expandable thumbnail
  • Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (AB323884), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (AB323884), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (AB323884), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (AB323884), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins (PubMed:34587386). Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).

Alternative names

C1orf139, GPR177, UNQ85/PRO18667, WLS, Protein wntless homolog, Integral membrane protein GPR177, Protein evenness interrupted homolog, Putative NF-kappa-B-activating protein 373, EVI

Rabbit Recombinant Monoclonal GPR177/WLS antibody. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29686-521
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884), expandable thumbnail

    Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884)

    GPR177/WLS Western blot staining using rabbit Anti-GPR177/WLS antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HepG2, K-562.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884) at 1/1000 dilution

    Lane 1: HaCaT (human skin keratinocyte) whole lysate at 60 µg

    Lane 2: A-204 (human muscle rhabdomyosarcoma cell) whole lysate at 60 µg

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole lysate at 60 µg

    Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole lysate at 60 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 124 kDa

    Exposure time: 15s

  • Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884), expandable thumbnail

    Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884)

    GPR177/WLS Western blot staining using rabbit Anti-GPR177/WLS antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 60 µg

    Lane 2: U-87 MG transfected with siRNA specifically targeting WLS whole cell lysate at 60 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884)

    GPR177/WLS Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-GPR177/WLS antibody

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GPR177/WLS with ab323884 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human kidney. The section was incubated with ab323884 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884)

    GPR177/WLS Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human breast carcinoma using rabbit Anti-GPR177/WLS antibody

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GPR177/WLS with ab323884 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human breast carcinoma. The section was incubated with ab323884 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884)

    GPR177/WLS Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon carcinoma using rabbit Anti-GPR177/WLS antibody

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GPR177/WLS with ab323884 at 1/100 (5.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon carcinoma. The section was incubated with ab323884 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR177/WLS antibody [EPR29686-521] (ab323884)

    GPR177/WLS Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GPR177/WLS antibody

    Immunohistochemical analysis of paraffin-embedded (A) HaCaT (Human skin keratinocyte) cell pellet, (B) A-204 (Human muscle rhabdomyosarcoma cell) cell pellet, (C) HepG2 (Human hepatocellular carcinoma epithelial cell) cell pellet, and (D) K562 (Human chronic myelogenous leukemia lymphoblast) cell pellet labeling GPR177/WLS with ab323884 at 1/1000 (0.501 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on HaCaT (image A) and A-204 (image B). No staining on HepG2 (image C) and K562 (image D). The section was incubated with ab323884 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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