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AB323481

Anti-GPR177/WLS antibody [EPR29686-569]

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Rabbit Recombinant Monoclonal GPR177/WLS antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

C1orf139, GPR177, UNQ85/PRO18667, WLS, Protein wntless homolog, Integral membrane protein GPR177, Protein evenness interrupted homolog, Putative NF-kappa-B-activating protein 373, EVI

3 Images
Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481)
  • WB

Supplier Data

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HepG2, K-562

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481) at 1/1000 dilution

Lane 1:

HaCaT (human skin keratinocyte) whole lysate at 50 µg

Lane 2:

A-204 (human muscle rhabdomyosarcoma cell) whole lysate at 50 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole lysate at 50 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa,124 kDa

false

Exposure time: 26s

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481)
  • WB

Supplier Data

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : F9, A20

The expression of WLS is downregulated in response to LGK974 treatment (PMID : 34406391).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 37 seconds, Lanes 4-5 : 114 seconds.

All lanes:

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481) at 1/1000 dilution

Lane 1:

mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole lysate at 50 µg

Lane 2:

F9 (mouse embryonal carcinoma epithelial cell) whole lysate at 50 µg

Lane 3:

A20 (mouse reticulum sarcoma B lymphocyte) whole lysate at 50 µg

Lane 4:

Untreated A375 (human malignant melanoma epithelial cell) whole lysate at 50 µg

Lane 5:

A375 treated with 10uM LGK974 for 96h whole lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa,36 kDa

false

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481)
  • WB

Supplier Data

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The band above the target band (45 kDa) is expected to be aggregation products.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

U-87 MG transfected with siRNA specifically targeting WLS whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa,36 kDa

false

Exposure time: 70s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29686-569

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for human IHC-P.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins (PubMed : 34587386). Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).
See full target information WLS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com