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Rabbit Recombinant Monoclonal GPR177/WLS antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481), expandable thumbnail
  • Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481), expandable thumbnail
  • Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (AB323481), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins (PubMed:34587386). Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).

Alternative names

C1orf139, GPR177, UNQ85/PRO18667, WLS, Protein wntless homolog, Integral membrane protein GPR177, Protein evenness interrupted homolog, Putative NF-kappa-B-activating protein 373, EVI

Rabbit Recombinant Monoclonal GPR177/WLS antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29686-569
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481), expandable thumbnail

    Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: F9, A20

    The expression of WLS is downregulated in response to LGK974 treatment (PMID: 34406391).

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 37 seconds, Lanes 4-5: 114 seconds.

    All lanes: Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481) at 1/1000 dilution

    Lane 1: mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole lysate at 50 µg with NFDM/TBST

    Lane 2: F9 (mouse embryonal carcinoma epithelial cell) whole lysate at 50 µg with NFDM/TBST

    Lane 3: A20 (mouse reticulum sarcoma B lymphocyte) whole lysate at 50 µg with NFDM/TBST

    Lane 4: Untreated A375 (human malignant melanoma epithelial cell) whole lysate at 50 µg with NFDM/TBST

    Lane 5: A375 treated with 10uM LGK974 for 96h whole lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 36 kDa

  • Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481), expandable thumbnail

    Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The band above the target band (45 kDa) is expected to be aggregation products.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg with NFDM/TBST

    Lane 2: U-87 MG transfected with siRNA specifically targeting WLS whole cell lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481), expandable thumbnail

    Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HepG2, K-562

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-GPR177/WLS antibody [EPR29686-569] (ab323481) at 1/1000 dilution

    Lane 1: HaCaT (human skin keratinocyte) whole lysate at 50 µg with NFDM/TBST

    Lane 2: A-204 (human muscle rhabdomyosarcoma cell) whole lysate at 50 µg with NFDM/TBST

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole lysate at 50 µg with NFDM/TBST

    Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 124 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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