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AB250614

Anti-GPR182 antibody [EPR14327] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GPR182 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ADMR, GPR182, G-protein coupled receptor 182

2 Images
Western blot - Anti-GPR182 antibody [EPR14327] - BSA and Azide free (AB250614)
  • WB

Supplier Data

Western blot - Anti-GPR182 antibody [EPR14327] - BSA and Azide free (AB250614)

This data was developed using ab182555, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GPR182 antibody [EPR14327] (<a href='/en-us/products/primary-antibodies/gpr182-antibody-epr14327-ab182555'>ab182555</a>) at 1/5000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Western blot - Anti-GPR182 antibody [EPR14327] - BSA and Azide free (AB250614)
  • WB

Supplier Data

Western blot - Anti-GPR182 antibody [EPR14327] - BSA and Azide free (AB250614)

This data was developed using ab182555, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GPR182 antibody [EPR14327] (<a href='/en-us/products/primary-antibodies/gpr182-antibody-epr14327-ab182555'>ab182555</a>) at 1/5000 dilution

Lane 1:

HT-1376 cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14327

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250614 is the carrier-free version of ab182555.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR182 also known by the alternate name adenylate cyclase inhibitory G protein-coupled receptor is a transmembrane protein weighing approximately 45 kDa. It belongs to the family of G protein-coupled receptors (GPCRs) which possess seven transmembrane domains. GPR182 is widely expressed in tissues such as the heart liver and kidney. Its expression suggests a role in various physiological processes where it might interact with other molecules across cellular membranes.
Biological function summary

GPR182 influences cellular signaling by potentially regulating cyclic AMP (cAMP) levels. This receptor does not appear to form part of a larger protein complex but operates independently in signal transduction. Its signaling activity might affect processes such as cell growth and differentiation which could imply roles in development and response to external signals. Through these processes GPR182 contributes to maintaining cellular homeostasis.

Pathways

GPR182 engages in pathways that regulate cAMP production. This engagement links it to the G protein signaling pathway important for transmitting signals from hormones and neurotransmitters. Related proteins in this pathway include the adenylate cyclases which produce cAMP and other GPCRs involved in similar signaling roles. These connections help modulate different physiological responses demonstrating the importance of GPR182 in cellular communication networks.

GPR182 shows relevance to cardiovascular disease and cancer. Its expression and function imply roles in growth and repair processes of cardiovascular tissues. In cancer GPR182 might interact with proteins like beta-catenin influencing cell proliferation and metastasis. These interactions suggest GPR182 as a potential target for therapeutic strategies aiming to regulate abnormal signaling and contribute to disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor.
See full target information GPR182
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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