Rabbit Recombinant Monoclonal G37L1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Transfected cell line - Mouse, Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433753.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625)")
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431971.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625)")
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625)")
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431969.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625)")
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431968.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (AB317625)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IHC-Fr | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended |
| Transfected cell line - Mouse | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell line - Mouse | Dilution info - | Notes - |
Target data
Function
G-protein coupled receptor (PubMed:27072655). Has been shown to bind the neuroprotective and glioprotective factor prosaposin (PSAP), leading to endocytosis followed by an ERK phosphorylation cascade (By similarity). However, other studies have shown that prosaposin does not increase activity (By similarity). It has been suggested that GPR37L1 is a constitutively active receptor which signals through the guanine nucleotide-binding protein G(s) subunit alpha (By similarity). Participates in the regulation of postnatal cerebellar development by modulating the Shh pathway (PubMed:24062445). Regulates baseline blood pressure in females and protects against cardiovascular stress in males (PubMed:29625592). Mediates inhibition of astrocyte glutamate transporters and reduction in neuronal N-methyl-D-aspartate receptor activity (PubMed:28795439).
Alternative names
Etbrlp2, G-protein coupled receptor 37-like 1, Endothelin B receptor-like protein 2, ETBR-LP-2
Rabbit Recombinant Monoclonal G37L1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Transfected cell line - Mouse, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29125-35
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317625 is the carrier-free version of Anti-GPR37L1 antibody [EPR29125-35] ab317624.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR37L1 also known as Prosaposin receptor GPR37L1 is an orphan G protein-coupled receptor with a mass of approximately 70 kDa. It is highly expressed in the central nervous system particularly in astrocytes and also in some peripheral tissues. The receptor is involved in signaling mechanisms through coupling with G proteins often leading to intracellular responses such as calcium mobilization or changes in cyclic AMP levels. Its expression pattern suggests a significant role in neural functions.
- Biological function summary
GPR37L1 participates in modulating astrocytic activities which are critical for maintaining neural homeostasis. Although it does not typically form part of large protein complexes it interacts with various intracellular signaling components to exert its effects. Researchers have suggested that GPR37L1 could influence neuroinflammatory processes and cell survival due to its presence in neural tissues where it might regulate responses to stress and environmental cues.
- Pathways
GPR37L1 plays a role in the Wnt signaling pathway and the MAPK signaling pathway both of which are essential for cellular growth differentiation and survival. These pathways involve interactions with other proteins like Frizzled receptors in the Wnt pathway and Ras proteins in the MAPK pathway. GPR37L1's contribution to these pathways highlights its importance in cellular communication and signal transduction potentially coordinating responses to different physiological and pathological stimuli.
- Associated diseases and disorders
GPR37L1 has been associated with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. This receptor's altered signaling might contribute to the pathophysiology of these disorders linking to proteins like alpha-synuclein and amyloid precursor protein respectively. Understanding the role of GPR37L1 in these diseases could offer new insights for therapeutic strategies aimed at modulating its activity to improve neuronal health and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img433753.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse GPR37L1 expression vector containing a Myc-His tag; (B) HEK-293T transfected with GPR37 vector expression containing a Myc-His tag; (C) HEK-293T transfected with empty vector expression containing a Myc-His tag tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/4000 (0.128 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse GPR37L1 expression vector containing a Myc-His tag, and no staining in (B) HEK-293T transfected with GPR37 vector expression containing a Myc-His tag and (C) HEK-293T transfected with empty vector expression containing a Myc-His tag. This antibody does not cross-react with mouse GPR37.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431971.jpg "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/50 (10.26 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431970.jpg "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/50 (10.26 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-GPR37L1 (Anti-GPR37L1 antibody [EPR29125-35] ab317624, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on rat cerebellum.
Panel B: anti-GPR37L1 stained on rat cerebellum.
Panel C: anti-NeuN stained in neurons of rat cerebellum.
Panel D: anti-GFAP stained in astrocytes of rat cerebellum.
The section was incubated in two rounds of staining: in the order of Anti-GPR37L1 antibody [EPR29125-35] ab317624 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431969.jpg "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/50 (10.26 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-frozen-sections-img431968.jpg "Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Frozen sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/50 (10.26 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-GPR37L1 (Anti-GPR37L1 antibody [EPR29125-35] ab317624, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on mouse cerebellum.
Panel B: anti-GPR37L1 stained on mouse cerebellum.
Panel C: anti-NeuN stained in neurons of mouse cerebellum.
Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
The section was incubated in two rounds of staining: in the order of Anti-GPR37L1 antibody [EPR29125-35] ab317624 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431914.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse liver.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431913.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in rat liver.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431912.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human liver.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431911.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in rat cardiac muscle.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431910.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse cardiac muscle.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431909.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human cardiac muscle.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431907.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat cerebellum.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431906.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse cerebellum.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431905.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)
This data was developed using Anti-GPR37L1 antibody [EPR29125-35] ab317624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling GPR37L1 with Anti-GPR37L1 antibody [EPR29125-35] ab317624 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human cerebellum.
The section was incubated with Anti-GPR37L1 antibody [EPR29125-35] ab317624 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431914.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431913.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431912.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431911.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431910.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431909.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431907.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431906.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625), expandable thumbnail](https://content.abcam.com/products/images/gpr37l1-antibody-epr29125-35-bsa-and-azide-free-ab317625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img431905.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR37L1 antibody [EPR29125-35] - BSA and Azide free (ab317625)")
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