AB100896

Anti-GPR54 antibody

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(7 Publications)

Rabbit Polyclonal GPR54 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

AXOR12, GPR54, KISS1R, KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, hOT7T175

1 Images

Unknown

Western blot - Anti-GPR54 antibody (AB100896)

GPR54 receptor contains a number of potential glycosylation sites (SwissProt) which may explain the migration pattern observed.

All lanes:

Western blot - Anti-GPR54 antibody (ab100896) at 1 µg/mL

All lanes:

Human spinal cord tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spinal-cord-tissue-lysate-total-protein-ab29188'>ab29188</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 42 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR54 also known as Kisspeptin Receptor is a G-protein coupled receptor with a molecular mass of approximately 42 kDa. Scientists typically find GPR54 expressed in the hypothalamus pituitary and gonads where it plays a mechanical role in signaling. This receptor interacts specifically with kisspeptin a peptide that binds to it and initiates a cascade of intracellular signaling events that regulate hormone release.

Biological function summary

GPR54 functions as a critical regulator of reproductive processes. When activated by its ligand kisspeptin GPR54 triggers the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus. This action promotes the secretion of luteinizing hormone and follicle-stimulating hormone. Both of these hormones are necessary for normal reproductive function. It is not part of a larger complex but plays an important role in its signaling pathway independent of complex formation.

Pathways

GPR54 is integral to the hypothalamic-pituitary-gonadal (HPG) axis. This pathway helps regulate sexual maturation and reproductive cycles in mammals. GPR54 by encouraging GnRH secretion and subsequent hormone release is essential for functioning the HPG axis. Moreover it also interfaces with signaling pathways related to metabolic regulation due to its broad influence on energy balance hormones. Related proteins in these pathways include GnRH itself and downstream signaling molecules like the LH and FSH receptors.

GPR54 links strongly to conditions such as hypogonadotropic hypogonadism and disorders of puberty onset. Mutations or dysregulation in GPR54 can lead to delayed or absent puberty due to the inadequate release of GnRH. Moreover researchers have noted connections between GPR54 and the protein LH as the absence or mutation of GPR54 disrupts LH production affecting overall reproductive health. This receptor's function positions it as a potential therapeutic target for treating reproductive disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

See full target information KISS1R

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 105:e70134 PubMed40454669

2025

Kisspeptin-54 Restores Blood-Brain Barrier Integrity via GATA-4 in Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Shan,Haiyan Lan,Yini Wu,Qiaomin Xu,Minji You,Ruijun Ma

CNS neuroscience & therapeutics 31:e70413 PubMed40384564

2025

Kisspeptin-10 Prevents the Development of Cerebral Aneurysms by Reducing the Expression of Egr-1.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Yu,Minghong Xie,Xuancong Liufu,Yezi Xu,Lei Chen

Molecular medicine reports 25: PubMed35234270

2022

LKB1 alleviates high glucose‑ and high fat‑induced inflammation and the expression of GnRH and sexual precocity‑related genes, in mouse hypothalamic cells by activating the AMPK/FOXO1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Limei Guan,Qing Zhou,Hailong Huang,Liangpu Xu

Reproductive biology and endocrinology : RB&E 19:12 PubMed33472656

2021

Leptin and inflammatory factors play a synergistic role in the regulation of reproduction in male mice through hypothalamic kisspeptin-mediated energy balance.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chang,Chenglin Song,Haining Gao,Tie Ma,Tao Li,Qianhe Ma,Tingting Yao,Meng Wang,Jing Li,Xuejie Yi,Donghui Tang,Shicheng Cao

Diabetes, metabolic syndrome and obesity : targets 13:4681-4688 PubMed33299335

2020

Mediates GnRH Secretion via System in GT1-7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Haiying Wu,Xiuli Chen,Rongrong Xie,Fengyun Wang,Hui Sun,Linqi Chen

Medical science monitor : international medical jo 26:e922860 PubMed32451371

2020

Effects of High-Glucose and High-Fat Condition on Estrogen Receptor- and Sexual Precocity-Related Genes in GT1-7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shuqin Wang,Hong Yao,Lan Ding,Yiqing Gao,Pei Wang,Ying Xue

The Journal of endocrinology 214:79-85 PubMed22526494

2012

Altered placental expression of kisspeptin and its receptor in pre-eclampsia.

Applications

Species

Human

Judith E Cartwright,Paula Juliet Williams

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GPR54 antibody

Western blot - Anti-GPR54 antibody (AB100896)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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