AB188995

Anti-GPR54 antibody - C-terminal

Name: Anti-GPR54 antibody - C-terminal (ab188995) | Abcam
Brand: Abcam Limited
SKU: AB188995

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(2 Publications)

Rabbit Polyclonal GPR54 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KISS1R.

View Alternative Names

AXOR12, GPR54, KISS1R, KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, hOT7T175

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (AB188995)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (AB188995)

Immunohistochemical analysis of formalin fixed, paraffin embedded human tonsil tissue labeling GPR54 receptor with ab188995 at 5 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (AB188995)

Immunohistochemical analysis of formalin fixed, paraffin embedded human brain cerebellum tissue labeling GPR54 receptor with ab188995 at 5 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human KISS1R. The exact immunogen used to generate this antibody is proprietary information.

Q969F8

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZC3H3 (50%).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR54 also known as Kisspeptin Receptor is a G-protein coupled receptor with a molecular mass of approximately 42 kDa. Scientists typically find GPR54 expressed in the hypothalamus pituitary and gonads where it plays a mechanical role in signaling. This receptor interacts specifically with kisspeptin a peptide that binds to it and initiates a cascade of intracellular signaling events that regulate hormone release.

Biological function summary

GPR54 functions as a critical regulator of reproductive processes. When activated by its ligand kisspeptin GPR54 triggers the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus. This action promotes the secretion of luteinizing hormone and follicle-stimulating hormone. Both of these hormones are necessary for normal reproductive function. It is not part of a larger complex but plays an important role in its signaling pathway independent of complex formation.

Pathways

GPR54 is integral to the hypothalamic-pituitary-gonadal (HPG) axis. This pathway helps regulate sexual maturation and reproductive cycles in mammals. GPR54 by encouraging GnRH secretion and subsequent hormone release is essential for functioning the HPG axis. Moreover it also interfaces with signaling pathways related to metabolic regulation due to its broad influence on energy balance hormones. Related proteins in these pathways include GnRH itself and downstream signaling molecules like the LH and FSH receptors.

GPR54 links strongly to conditions such as hypogonadotropic hypogonadism and disorders of puberty onset. Mutations or dysregulation in GPR54 can lead to delayed or absent puberty due to the inadequate release of GnRH. Moreover researchers have noted connections between GPR54 and the protein LH as the absence or mutation of GPR54 disrupts LH production affecting overall reproductive health. This receptor's function positions it as a potential therapeutic target for treating reproductive disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

See full target information KISS1R

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 13:31 PubMed40533847

2025

Obesity and cervical intraepithelial neoplasia: regulation of mitochondrial energy metabolism via the Kisspeptin/GPR54 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Pan,Yuanyuan Chen,Yan Ye,Peipei Li,Feifei Ni,Haizhen He

Frontiers in endocrinology 12:652807 PubMed33868179

2021

The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Meihe Li,Yang Haixia,Minchao Kang,Peng An,Xili Wu,Huimin Dang,Xin Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPR54 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (AB188995)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (AB188995)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise