Rabbit Polyclonal GPR54 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KISS1R.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KISS1R. The exact immunogen used to generate this antibody is proprietary information. Database link Q969F8
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.00000-5.00000 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.
Alternative names
AXOR12, GPR54, KISS1R, KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, hOT7T175
Recommended products
Rabbit Polyclonal GPR54 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KISS1R.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KISS1R. The exact immunogen used to generate this antibody is proprietary information. Database link Q969F8
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZC3H3 (50%).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR54 also known as Kisspeptin Receptor is a G-protein coupled receptor with a molecular mass of approximately 42 kDa. Scientists typically find GPR54 expressed in the hypothalamus pituitary and gonads where it plays a mechanical role in signaling. This receptor interacts specifically with kisspeptin a peptide that binds to it and initiates a cascade of intracellular signaling events that regulate hormone release.
- Biological function summary
GPR54 functions as a critical regulator of reproductive processes. When activated by its ligand kisspeptin GPR54 triggers the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus. This action promotes the secretion of luteinizing hormone and follicle-stimulating hormone. Both of these hormones are necessary for normal reproductive function. It is not part of a larger complex but plays an important role in its signaling pathway independent of complex formation.
- Pathways
GPR54 is integral to the hypothalamic-pituitary-gonadal (HPG) axis. This pathway helps regulate sexual maturation and reproductive cycles in mammals. GPR54 by encouraging GnRH secretion and subsequent hormone release is essential for functioning the HPG axis. Moreover it also interfaces with signaling pathways related to metabolic regulation due to its broad influence on energy balance hormones. Related proteins in these pathways include GnRH itself and downstream signaling molecules like the LH and FSH receptors.
- Associated diseases and disorders
GPR54 links strongly to conditions such as hypogonadotropic hypogonadism and disorders of puberty onset. Mutations or dysregulation in GPR54 can lead to delayed or absent puberty due to the inadequate release of GnRH. Moreover researchers have noted connections between GPR54 and the protein LH as the absence or mutation of GPR54 disrupts LH production affecting overall reproductive health. This receptor's function positions it as a potential therapeutic target for treating reproductive disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (ab188995)
Immunohistochemical analysis of formalin fixed, paraffin embedded human tonsil tissue labeling GPR54 receptor with ab188995 at 5 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR54 antibody - C-terminal (ab188995)
Immunohistochemical analysis of formalin fixed, paraffin embedded human brain cerebellum tissue labeling GPR54 receptor with ab188995 at 5 μg/ml.
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