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AB259820

Anti-GPR56 antibody [EPR23908-109]

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Rabbit Recombinant Monoclonal GPR56 antibody. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

GPR56, TM7LN4, TM7XN1, UNQ540/PRO1083, ADGRG1, Adhesion G-protein coupled receptor G1, G-protein coupled receptor 56

1 Images
Flow Cytometry - Anti-GPR56 antibody [EPR23908-109] (AB259820)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-GPR56 antibody [EPR23908-109] (AB259820)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling GPR56 with ab259820 at 1/50 dilution (1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG or ab259820. Then stained with anti-CD56 conjugated to BV421.

Gated on viable cells.

  • Carrier free

    Anti-GPR56 antibody [EPR23908-109] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23908-109

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

This antibody ab259820 [EPR23908-109] is a different clone to ab274419 [EPR23908-115].

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR56 also known as ADGRG1 is a G protein-coupled receptor. It has a mass of around 75 kDa. It plays an essential role in cellular signaling. Found in various tissues GPR56 is expressed significantly in brain and prostate tissue. This receptor is part of the adhesion GPCR family characterized by its large N-terminal region which is involved in cell adhesion and interaction. The mechanical functions of GPR56 involve regulating cell communication which influences cellular growth and behavior.
Biological function summary

GPR56 participates in brain development particularly in the formation of the cerebral cortex. It interacts with extracellular matrix components like collagen III which impacts cell adhesion and migration processes. GPR56 does not commonly form complexes but interacts with other cell membrane molecules to carry out its biological roles. Research indicates its involvement in cellular processes such as cell signaling pathways and the maintenance of tissue integrity.

Pathways

GPR56 is involved in both the inside-out and outside-in signaling pathways. In these pathways GPR56 modulates cell adhesion and migration dynamics. Related proteins such as collagen III interact with GPR56 to facilitate these processes. Furthermore the receptor interacts with integrin signaling pathways that help regulate the homeostasis of the extracellular matrix proving critical in maintaining normal cellular physiology.

Mutations or dysregulation of GPR56 have been associated with brain malformations such as bilateral frontoparietal polymicrogyria. It also plays a role in certain cancers including melanoma where its function in cell adhesion contributes to metastasis. Researchers study its relationship with proteins like integrins which when altered may contribute to cancer progression. Understanding GPR56's roles could offer new insights into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adhesion G-protein coupled receptor (aGPCR) for steroid hormone 17alpha-hydroxypregnenolone (17-OH), which is involved in cell adhesion and cell-cell interactions (PubMed : 39389061). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as RhoA pathway (PubMed : 28874577, PubMed : 35418682, PubMed : 39389061). ADGRG1 is coupled to G(12) and/or G(13) G proteins (GNA12 and GNA13, respectively) and mediates the activation Rho small GTPases (PubMed : 22238662, PubMed : 28424266, PubMed : 35418682, PubMed : 39389061). Acts as a potent suppressor of ferroptosis : binding to 17-OH-binding initiates signaling that down-regulates CD36 and alleviates ferroptosis-induced liver injury (By similarity). Ligand-binding also induces cell adhesion activity via association with proteins such as collagen III/COL3A1 and TGM2 (By similarity). Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells (By similarity). Involved in cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination : association with COL3A1 in the developing brain inhibits neuronal migration via activation of the RhoA pathway (PubMed : 24531968). Together with TGM2, acts as a regulator of myelination and myelin repair in oligodendrocyte precursor cells (By similarity). Acts as a hemostatic sensor of shear force : G protein-coupled receptor signaling is activated in response to shear force in platelets, promoting G(13) G protein signaling, and platelet shape change and aggregation in a COL3A1-dependent manner (PubMed : 33097663). Acts as an inhibitor of VEGFA production thereby inhibiting angiogenesis through a signaling pathway mediated by PRKCA (PubMed : 16757564, PubMed : 19572147, PubMed : 21724588). Plays a role in the maintenance of hematopoietic stem cells in bone marrow niche (By similarity). Plays an essential role in testis development (By similarity).
See full target information ADGRG1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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