AB274429

Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GPR56 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GPR56, TM7LN4, TM7XN1, UNQ540/PRO1083, ADGRG1, Adhesion G-protein coupled receptor G1, G-protein coupled receptor 56

2 Images

Lab

Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (AB274429)

This data was developed using ab274419, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 17932623).

Negative control : Human lung (PMID : 15674329)

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-GPR56 antibody [EPR23908-115] (<a href='/en-us/products/primary-antibodies/gpr56-antibody-epr23908-115-ab274419'>ab274419</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 40 µg

Lane 2:

Human hippocampus tissue lysate at 40 µg

Lane 3:

Human lung tissue lysate at 40 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 78 kDa

Observed band size: 65 kDa

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Lab

Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (AB274429)

This data was developed using ab274419, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 17932623).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 70 seconds.

All lanes:

Western blot - Anti-GPR56 antibody [EPR23908-115] (<a href='/en-us/products/primary-antibodies/gpr56-antibody-epr23908-115-ab274419'>ab274419</a>) at 1/1000 dilution

All lanes:

Human glioma tissue lysate at 10 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 78 kDa

Observed band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23908-115

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This antibody ab274429 [EPR23908-115] is a different clone to ab274428 [EPR23908-109].

ab274429 is the carrier-free version of ab274419.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR56 also known as ADGRG1 is a G protein-coupled receptor. It has a mass of around 75 kDa. It plays an essential role in cellular signaling. Found in various tissues GPR56 is expressed significantly in brain and prostate tissue. This receptor is part of the adhesion GPCR family characterized by its large N-terminal region which is involved in cell adhesion and interaction. The mechanical functions of GPR56 involve regulating cell communication which influences cellular growth and behavior.

Biological function summary

GPR56 participates in brain development particularly in the formation of the cerebral cortex. It interacts with extracellular matrix components like collagen III which impacts cell adhesion and migration processes. GPR56 does not commonly form complexes but interacts with other cell membrane molecules to carry out its biological roles. Research indicates its involvement in cellular processes such as cell signaling pathways and the maintenance of tissue integrity.

Pathways

GPR56 is involved in both the inside-out and outside-in signaling pathways. In these pathways GPR56 modulates cell adhesion and migration dynamics. Related proteins such as collagen III interact with GPR56 to facilitate these processes. Furthermore the receptor interacts with integrin signaling pathways that help regulate the homeostasis of the extracellular matrix proving critical in maintaining normal cellular physiology.

Mutations or dysregulation of GPR56 have been associated with brain malformations such as bilateral frontoparietal polymicrogyria. It also plays a role in certain cancers including melanoma where its function in cell adhesion contributes to metastasis. Researchers study its relationship with proteins like integrins which when altered may contribute to cancer progression. Understanding GPR56's roles could offer new insights into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adhesion G-protein coupled receptor (aGPCR) for steroid hormone 17alpha-hydroxypregnenolone (17-OH), which is involved in cell adhesion and cell-cell interactions (PubMed : 39389061). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as RhoA pathway (PubMed : 28874577, PubMed : 35418682, PubMed : 39389061). ADGRG1 is coupled to G(12) and/or G(13) G proteins (GNA12 and GNA13, respectively) and mediates the activation Rho small GTPases (PubMed : 22238662, PubMed : 28424266, PubMed : 35418682, PubMed : 39389061). Acts as a potent suppressor of ferroptosis : binding to 17-OH-binding initiates signaling that down-regulates CD36 and alleviates ferroptosis-induced liver injury (By similarity). Ligand-binding also induces cell adhesion activity via association with proteins such as collagen III/COL3A1 and TGM2 (By similarity). Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells (By similarity). Involved in cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination : association with COL3A1 in the developing brain inhibits neuronal migration via activation of the RhoA pathway (PubMed : 24531968). Together with TGM2, acts as a regulator of myelination and myelin repair in oligodendrocyte precursor cells (By similarity). Acts as a hemostatic sensor of shear force : G protein-coupled receptor signaling is activated in response to shear force in platelets, promoting G(13) G protein signaling, and platelet shape change and aggregation in a COL3A1-dependent manner (PubMed : 33097663). Acts as an inhibitor of VEGFA production thereby inhibiting angiogenesis through a signaling pathway mediated by PRKCA (PubMed : 16757564, PubMed : 19572147, PubMed : 21724588). Plays a role in the maintenance of hematopoietic stem cells in bone marrow niche (By similarity). Plays an essential role in testis development (By similarity).

See full target information ADGRG1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free

Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (AB274429)

Western blot - Anti-GPR56 antibody [EPR23908-115] - BSA and Azide free (AB274429)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise