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AB237610

Anti-GPR83 antibody

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(1 Publication)

Rabbit Polyclonal GPR83 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GPR83 aa 300 to C-terminus.

View Alternative Names

GPR72, JP05, KIAA1540, GPR83, G-protein coupled receptor 83, G-protein coupled receptor 72

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPR83 antibody (AB237610)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPR83 antibody (AB237610)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for GPR83 (green) using ab237610 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-GPR83 antibody (AB237610)
  • WB

Supplier Data

Western blot - Anti-GPR83 antibody (AB237610)

All lanes:

Western blot - Anti-GPR83 antibody (ab237610) at 1/1000 dilution

All lanes:

LO2 (Human immortal liver cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GPR83 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NYM4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR83 also known as GIR or G-protein coupled receptor 83 is a member of the class A rhodopsin-like family of G-protein coupled receptors (GPCRs). This receptor has a mass of approximately 47 kDa. GPR83 is expressed in several tissues with significant expression in the brain particularly in the hypothalamus and to a lesser extent in the cortex and brainstem. It is also found in immune system tissues like the thymus and lymph nodes suggesting roles in both neurological and immune functions.
Biological function summary

GPR83 engages in the regulation of neuroendocrine signaling. There is evidence that it affects appetite control and energy homeostasis implying a role in metabolic processes. GPR83 does not function as part of a larger receptor complex but interacts with other GPCRs or intracellular proteins to transmit extracellular signals into cells. Its activity can modulate synaptic transmission and impact behaviors linked to feeding and stress response.

Pathways

Protein interactions involving GPR83 connect mainly to neuropeptide Y (NPY) signaling and the broader GPCR signaling pathways. The receptor modulates signaling by potentially influencing second messenger systems such as cAMP. Proteins related to these pathways include NPY a neuropeptide that affects energy balance and various G-protein subunits that mediate downstream effects following GPR83 activation.

Altered GPR83 expression or signaling links to obesity and certain psychiatric conditions including anxiety-related disorders. High GPR83 activity has associations with increased fat storage and body weight potentially by downregulating activity through pathways with proteins like NPY and agouti-related peptide (AgRP) which are involved in appetite control. Additionally imbalance in GPR83 signaling might influence mood disorders linked to dysregulation of neural circuits involved in stress and emotion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for PEN, a neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Acts through a G(i)- and G(q)-alpha-alpha-mediated pathway in response to PEN (PubMed : 27117253). Plays a role in food intake and body weight regulation. May contribute to the regulation of anxiety-related behaviors (By similarity).
See full target information GPR83
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298473

2023

Chondrocyte Thrombomodulin Protects against Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Kang,Ai-Lun Yang,Chao-Han Lai,Tsan-Ju Chen,Sung-Yen Lin,Yan-Hsiung Wang,Chau-Zen Wang,Edward M Conway,Hua-Lin Wu,Mei-Ling Ho,Je-Ken Chang,Chung-Hwan Chen,Tsung-Lin Cheng
View all publications
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Product promise

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