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AB325861

Anti-GPRC5A antibody [HL1864] - BSA and Azide free

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Rabbit Recombinant Monoclonal GPRC5A antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GPRC5A.

View Alternative Names

GPCR5A, RAI3, RAIG1, GPRC5A, Retinoic acid-induced protein 3, G-protein coupled receptor family C group 5 member A, Phorbol ester induced gene 1, Retinoic acid-induced gene 1 protein, PEIG-1, RAIG-1

13 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunofluorescent analysis. Sample : Wild-type (WT) and GPRC5A knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green : GPRC5A stained by ab325861 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. GPRC5A stained by ab325861 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human kidney. GPRC5A stained by ab325861 diluted at 1 : 100 and competitor's antibody diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein at cell membrane by immunofluorescent analysis. Sample : MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green : GPRC5A stained by ab325861 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human lung cancer. GPRC5A stained by ab325861 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. GPRC5A stained by ab325861 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325861 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/1000 dilution

Lane 1:

MCF7 whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325861 diluted at 1 : 5000 and competitor's antibody diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/5000 dilution

Lane 1:

Boiled wild-type (WT) MCF-7 cell extracts at 30 µg

Lane 2:

Boiled GPRC5A knockout (KO) MCF-7 cell extracts at 30 µg

Lane 3:

Unboiled wild-type (WT) MCF-7 cell extracts at 30 µg

Lane 4:

Unboiled GPRC5A knockout (KO) MCF-7 cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325861 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325861 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/1000 dilution

Lane 1:

Unboiled wild-type (WT) MCF-7 cell extracts at 30 µg

Lane 2:

Unboiled GPRC5A knockout (KO) MCF-7 cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325861 diluted at 1 : 50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/50000 dilution

Lanes 1 and 3:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled GPRC5a transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled GPRC5C transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325861 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/1000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tag tagged GPRC5A transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tag tagged GPRC5A transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Non-transfected (–) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ab325861 diluted at 1 : 5000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/5000 dilution

Lane 1:

Non-transfected (–) unboiled MCF-7 whole cell extracts at 30 µg

Lane 2:

GPRC5A shRNA transfected (+) unboiled MCF-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1864

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GPRC5A.

Q8NFJ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor (PubMed : 18000218).
See full target information GPRC5A
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Product promise

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For full details, please see our Terms & Conditions

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