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AB325862

Anti-GPRC5A antibody [HL3393] - BSA and Azide free

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Rabbit Recombinant Monoclonal GPRC5A antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples. Immunogen corresponding to Synthetic Peptide within Human GPRC5A.

View Alternative Names

GPCR5A, RAI3, RAIG1, GPRC5A, Retinoic acid-induced protein 3, G-protein coupled receptor family C group 5 member A, Phorbol ester induced gene 1, Retinoic acid-induced gene 1 protein, PEIG-1, RAIG-1

4 Images
Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325862 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (ab325862) at 1/1000 dilution

Lane 1:

A431whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)

Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325862 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (ab325862) at 1/1000 dilution

Lane 1:

Boiled wild-type (WT) MCF-7 cell extracts at 30 µg

Lane 2:

Boiled GPRC5A knockout (KO) MCF-7 cell extracts at 30 µg

Lane 3:

Unboiled wild-type (WT) MCF-7 cell extracts at 30 µg

Lane 4:

Unboiled GPRC5A knockout (KO) MCF-7 cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325862 diluted at 1 : 50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (ab325862) at 1/50000 dilution

Lanes 1 and 3:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled GPRC5A transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled GPRC5C transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)
  • WB

Supplier Data

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (AB325862)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325862 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL3393] - BSA and Azide free (ab325862) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged GPRC5A transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged GPRC5A transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL3393

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GPRC5A.

Q8NFJ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor (PubMed : 18000218).
See full target information GPRC5A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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