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AB232963

Anti-GPT2 antibody

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(3 Publications)

Rabbit Polyclonal GPT2 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GPT2 aa 200-300.

View Alternative Names

AAT2, ALT2, GPT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody (AB232963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody (AB232963)

Formalin-fixed, paraffin-embedded human kidney tissue stained for GPT2 using ab232963 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-GPT2 antibody (AB232963)
  • WB

Supplier Data

Western blot - Anti-GPT2 antibody (AB232963)

All lanes:

Western blot - Anti-GPT2 antibody (ab232963) at 1 µg/mL

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Western blot - Anti-GPT2 antibody (AB232963)
  • WB

Supplier Data

Western blot - Anti-GPT2 antibody (AB232963)

All lanes:

Western blot - Anti-GPT2 antibody (ab232963) at 2 µg/mL

All lanes:

Recombinant human GPT2 protein

Predicted band size: 58 kDa

false

Western blot - Anti-GPT2 antibody (AB232963)
  • WB

Supplier Data

Western blot - Anti-GPT2 antibody (AB232963)

All lanes:

Western blot - Anti-GPT2 antibody (ab232963) at 2 µg/mL

All lanes:

Pig skeletal muscle lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Western blot - Anti-GPT2 antibody (AB232963)
  • WB

Supplier Data

Western blot - Anti-GPT2 antibody (AB232963)

All lanes:

Western blot - Anti-GPT2 antibody (ab232963) at 1 µg/mL

All lanes:

Human liver lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Western blot - Anti-GPT2 antibody (AB232963)
  • WB

Supplier Data

Western blot - Anti-GPT2 antibody (AB232963)

All lanes:

Western blot - Anti-GPT2 antibody (ab232963) at 1 µg/mL

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GPT2 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q8TD30

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPT2 protein also known as Glutamyl-Prolyl-tRNA Synthetase 2 is an enzyme essential for the translation process in protein biosynthesis. It functions by charging tRNA molecules with their respective amino acids specifically glutamic acid and proline. The GPT2 protein has a mass of approximately 170 kDa and is expressed in various tissues including muscles and kidneys. This widespread expression suggests its significant role in maintaining protein synthesis in different cellular environments.
Biological function summary

GPT2 enzyme plays an important role in catalyzing the attachment of amino acids to their corresponding tRNA molecules a vital step in ribosomal protein synthesis. As part of the aminoacyl-tRNA synthetases GPT2 forms a complex with other synthetases to ensure accurate decoding of mRNA during translation. These complexes are essential for preserving translational fidelity across diverse biological systems impacting numerous cellular functions.

Pathways

The GPT2 enzyme integrates into the translation pathway where it ensures efficient and precise protein synthesis. Its activity connects closely with the mTOR signaling pathway which regulates cell growth and metabolism in response to nutritional status. GPT2 interacts with other tRNA synthetases and translation initiation factors forming a critical component of the protein synthesis mechanism. This highlights its coordination with other proteins to uphold proper cellular function.

GPT2 has associations with certain mitochondrial diseases due to defects in protein synthesis and metabolism. Errors or mutations in tRNA synthetases including GPT2 can lead to mitochondrial dysfunction and related disorders such as Charcot-Marie-Tooth disease. This connection implicates GPT2 and other proteins in its group like glycyl-tRNA synthetase in these neuropathological conditions. Understanding these associations can facilitate the development of targeted therapies for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
See full target information GPT2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 12:1647017 PubMed41019540

2025

Taohong Siwu Decoction alleviates high salt-induced calcium overload and ferroptosis in vascular endothelial cells in hypertension by regulating ATF4.

Applications

Unspecified application

Species

Unspecified reactive species

Lai Kwan Lam,Peng-Li Xu,Peng-Cheng Xie,Qiu-Er Liang,Ting Xie,Lee Yam Poon,Ya Xiao,Li-Guo Chen

PLoS pathogens 20:e1012305 PubMed38905309

2024

PoRVA G9P[23] and G5P[7] infections differentially promote PEDV replication by reprogramming glutamine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Haixin Liu,Haolun Tian,Pengcheng Hao,Huimin Du,Kun Wang,Yudong Qiu,Xiangrui Yin,Nana Wu,Qian Du,Dewen Tong,Yong Huang

Cancer & metabolism 8:23 PubMed33101674

2020

Metabolic plasticity of IDH1 glioma cell lines is responsible for low sensitivity to glutaminase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Ruiz-Rodado,Adrian Lita,Tyrone Dowdy,Orieta Celiku,Alejandra Cavazos Saldana,Herui Wang,Chun Zhang Yang,Raj Chari,Aiguo Li,Wei Zhang,Hua Song,Meili Zhang,Susie Ahn,Dionne Davis,Xiang Chen,Zhengping Zhuang,Christel Herold-Mende,Kylie J Walters,Mark R Gilbert,Mioara Larion
View all publications
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