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AB69279

Anti-GPT2 antibody

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Mouse Polyclonal GPT2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GPT2.

View Alternative Names

AAT2, ALT2, GPT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2

1 Images
Western blot - Anti-GPT2 antibody (AB69279)
  • WB

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Western blot - Anti-GPT2 antibody (AB69279)

All lanes:

Western blot - Anti-GPT2 antibody (ab69279) at 1/500 dilution

Lane 1:

GPT2 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GPT2.

Q8TD30

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPT2 protein also known as Glutamyl-Prolyl-tRNA Synthetase 2 is an enzyme essential for the translation process in protein biosynthesis. It functions by charging tRNA molecules with their respective amino acids specifically glutamic acid and proline. The GPT2 protein has a mass of approximately 170 kDa and is expressed in various tissues including muscles and kidneys. This widespread expression suggests its significant role in maintaining protein synthesis in different cellular environments.
Biological function summary

GPT2 enzyme plays an important role in catalyzing the attachment of amino acids to their corresponding tRNA molecules a vital step in ribosomal protein synthesis. As part of the aminoacyl-tRNA synthetases GPT2 forms a complex with other synthetases to ensure accurate decoding of mRNA during translation. These complexes are essential for preserving translational fidelity across diverse biological systems impacting numerous cellular functions.

Pathways

The GPT2 enzyme integrates into the translation pathway where it ensures efficient and precise protein synthesis. Its activity connects closely with the mTOR signaling pathway which regulates cell growth and metabolism in response to nutritional status. GPT2 interacts with other tRNA synthetases and translation initiation factors forming a critical component of the protein synthesis mechanism. This highlights its coordination with other proteins to uphold proper cellular function.

GPT2 has associations with certain mitochondrial diseases due to defects in protein synthesis and metabolism. Errors or mutations in tRNA synthetases including GPT2 can lead to mitochondrial dysfunction and related disorders such as Charcot-Marie-Tooth disease. This connection implicates GPT2 and other proteins in its group like glycyl-tRNA synthetase in these neuropathological conditions. Understanding these associations can facilitate the development of targeted therapies for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.
See full target information GPT2
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