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Rabbit Recombinant Monoclonal GPT2 antibody. Suitable for Dot, ICC/IF, IHC-P, WB, mIHC and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Multiplex immunohistochemistry - Anti-GPT2 antibody [EPR29176-18] (AB322261), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-GPT2 antibody [EPR29176-18] (AB322261), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GPT2 antibody [EPR29176-18] (AB322261), expandable thumbnail
  • Western blot - Anti-GPT2 antibody [EPR29176-18] (AB322261), expandable thumbnail
  • Dot Blot - Anti-GPT2 antibody [EPR29176-18] (AB322261), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotICC/IFIHC-PWBmIHC
Human
Expected
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Not recommended
Tested
Expected
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.

Alternative names

Rabbit Recombinant Monoclonal GPT2 antibody. Suitable for Dot, ICC/IF, IHC-P, WB, mIHC and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29176-18

Purification technique

Affinity purification Protein A

Specificity

Not suitable for mouse IHC-P

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Multiplex immunohistochemistry - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Multiplex immunohistochemistry - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human kidney staining of GPT2 with ab322261 at a 1/2000 (0.261 µg/ml) dilution and COX IV with Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 at 1/500 (0.228 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-GPT2 (green; Opal™570) and anti-COX IV (magenta; Opal™520) on human kidney.

    Panel B: anti-GPT2 showed granular staining in human kidney.

    Panel C: ant-COX IV showed granular staining in human kidney.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322261 and Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Multiplex immunohistochemistry - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human cardiac muscle staining of GPT2 with ab322261 at a 1/2000 (0.261 µg/ml) dilution and COX IV with Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 at 1/500 (0.228 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-GPT2 (green; Opal™570) and anti-COX IV (magenta; Opal™520) on human cardiac muscle.

    Panel B: anti-GPT2 showed granular staining in human cardiac muscle.

    Panel C: ant-COX IV showed granular staining in human cardiac muscle.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322261 and Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling GPT2 with ab322261 at 1/500 (1.044 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing mitochondrial staining in HepG2 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/500 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab322261 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody preadsorbed at 1/1000 2 ug/ml dilution
    -ve control 2:anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/500 5ug/ml dilution , followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

  • Western blot - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Western blot - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the higher MW bands in lanes 2-4 are unknown.

    All lanes: Western blot - Anti-GPT2 antibody [EPR29176-18] (ab322261) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 3: Mouse heart tissue lysate at 20 µg

    Lane 4: Human heart tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 46 kDa

    Exposure time: 136s

  • Dot Blot - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Dot Blot - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Dot blot analysis of GPT2 using ab322261 at 1:1000 (0.522 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: His-tagged human GPT1 fragment

    Lane2: His-tagged human GPT2 fragment

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human GPT1.

    #In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-GPT2 antibody [EPR29176-18] (ab322261) at 1/1000 dilution

    Lane 1: His-tagged human GPT1 fragment

    Lane 2: His-tagged human GPT2 fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling GPT2 with ab322261 at 1/500 (1.044 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing mitochondrial staining in C2C12 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/500 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab322261 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody preadsorbed at 1/1000 2 ug/ml dilution
    -ve control 2:anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/500 5ug/ml dilution , followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GPT2 with ab322261 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human kidney.

    The section was incubated with ab322261 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GPT2 with ab322261 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human liver.

    The section was incubated with ab322261 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling GPT2 with ab322261 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cardiac muscle (PMID: 17826732, PMID: 19360321).

    The section was incubated with ab322261 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPT2 antibody [EPR29176-18] (ab322261)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GPT2 with ab322261 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: No staining on human cerebrum (PMID: 17826732).

    The section was incubated with ab322261 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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