Rabbit Recombinant Monoclonal GPVI antibody. Carrier free. Suitable for IHC-P, IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--flow-cytometry-img170343.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169855.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987)")
![Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-frozen-sections-img175575.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987)")
![Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunoprecipitation-img182752.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987)")
![Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--western-blot-img172310.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (AB289987)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Target data
Function
Collagen receptor involved in collagen-induced platelet adhesion and activation (PubMed:16139873, PubMed:24368846). Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely FYN or LYN) and SYK, the adapter protein LAT and leads to the activation of PLCG2.
Alternative names
Platelet glycoprotein VI, GPVI, Glycoprotein 5, Gp6
Recommended products
Rabbit Recombinant Monoclonal GPVI antibody. Carrier free. Suitable for IHC-P, IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25283-14
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab289987 is the carrier-free version of Anti-GPVI antibody [EPR25283-14] ab289964.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPVI or glycoprotein VI functions mechanically as a collagen receptor on the surface of platelets. With a molecular mass around 62 kDa it is expressed mainly on megakaryocytes and platelets. GPVI plays an important role in platelet adhesion and activation by binding to collagen exposed at sites of vascular injury. This interaction initiates a cascade of platelet activation essential for normal hemostasis.
- Biological function summary
The target participates in the formation of platelet activation complexes. GPVI partners with the Fc receptor γ-chain to transmit activation signals upon collagen binding. This process results in platelet shape change granule secretion and aggregation which are all critical steps for clot formation. By interacting with collagen GPVI ensures that platelets can effectively respond to vascular damage particularly in arterial vessels where high shear forces prevail.
- Pathways
The glycoprotein VI contributes significantly to the immune and coagulation pathways. It activates the enzyme Syk kinase following collagen binding which further triggers the downstream signaling pathways like the MAPK pathway leading to platelet activation. GPVI works closely with other receptors like integrin α2β1 and the glycoprotein Ib/V/IX complex reinforcing platelet-collagen interactions and stabilizing the platelet plug formation during thrombus growth.
- Associated diseases and disorders
Glycoprotein VI shows relevance in cardiovascular diseases and immune thrombocytopenia. Abnormal GPVI function or expression can exacerbate conditions such as myocardial infarction by promoting excessive platelet activation and thrombosis. Conversely reduced GPVI levels relate to bleeding disorders due to impaired platelet function. Studies have also linked GPVI to autoimmune conditions where antibodies may target it similar to responses in immune thrombocytopenia.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--flow-cytometry-img170343.jpg "Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Flow Cytometry - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using Anti-GPVI antibody [EPR25283-14] ab289964, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse blood cells cells labelling GPVI with Anti-GPVI antibody [EPR25283-14] ab289964 at 1/500 dilution (0.1μg)(Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used as secondary antibody at 1/2000 dilution. Cells were stained with rabbit IgG or Anti-GPVI antibody [EPR25283-14] ab289964. Then stained with anti-CD41 conjμgated to APC. Gated on viable cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169855.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using Anti-GPVI antibody [EPR25283-14] ab289964, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling GPVI with Anti-GPVI antibody [EPR25283-14] ab289964 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on megakaryocytes and platelets of mouse spleen. The section was incubated with Anti-GPVI antibody [EPR25283-14] ab289964 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
![Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-frozen-sections-img175575.jpg "Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using Anti-GPVI antibody [EPR25283-14] ab289964, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling GPVI with Anti-GPVI antibody [EPR25283-14] ab289964 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining in megakaryocytes of mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunoprecipitation-img182752.jpg "Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using Anti-GPVI antibody [EPR25283-14] ab289964, the same antibody clone in a different buffer formulation.
GPVI was immunoprecipitated from Mouse platelet lysate with Anti-GPVI antibody [EPR25283-14] ab289964 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GPVI antibody [EPR25283-14] ab289964 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse platelet lysate 10 μg
Lane 2: Anti-GPVI antibody [EPR25283-14] ab289964 IP in Mouse platelet lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GPVI antibody [EPR25283-14] ab289964 in mouse platelet lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
The expression profile/ molecμlar weight observed is consistent with what has been described in the literature (PMID: 23112346, PMID: 9295288).
All lanes: Immunoprecipitation - Anti-GPVI antibody [EPR25283-14] (Anti-GPVI antibody [EPR25283-14] ab289964)
Predicted band size: 37 kDa
Observed band size: 58 kDa
![Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--western-blot-img172310.jpg "Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using 289964, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23112346, 16014566).
Negative control: cerebellum. (PMID: 10961879).
Exposure time: 70 seconds.
All lanes: Western blot - Anti-GPVI antibody [EPR25283-14] (Anti-GPVI antibody [EPR25283-14] ab289964) at 1/1000 dilution
Lane 1: Mouse platelet lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 58 kDa
![Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--western-blot-img170509.jpg "Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using 289964, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:�23112346, PMID:�16014566 ).
114 seconds
Exposure time:
All lanes: Western blot - Anti-GPVI antibody [EPR25283-14] (Anti-GPVI antibody [EPR25283-14] ab289964) at 1/1000 dilution
Lane 1: Mouse platelet treated with PNGase F lysate at 20 µg
Lane 2: Mouse platelet lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 34 kDa, 58 kDa
![Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-frozen-sections-img169862.jpg "Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using Anti-GPVI antibody [EPR25283-14] ab289964, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GPVI with Anti-GPVI antibody [EPR25283-14] ab289964 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control (PMID: 10961879). No staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166679.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)
This data was developed using Anti-GPVI antibody [EPR25283-14] ab289964, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling GPVI with Anti-GPVI antibody [EPR25283-14] ab289964 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: no staining on mouse cerebrum PMID 10961879). The section was incubated with Anti-GPVI antibody [EPR25283-14] ab289964 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
![Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--western-blot-img170509.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
![Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-frozen-sections-img169862.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987), expandable thumbnail](https://content.abcam.com/products/images/gpvi-antibody-epr25283-14-bsa-and-azide-free-ab289987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166679.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPVI antibody [EPR25283-14] - BSA and Azide free (ab289987)")
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