AB96257

Anti-GPx-7 antibody

Name: Anti-GPx-7 antibody (ab96257) | Abcam
Brand: Abcam Limited
SKU: AB96257
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GPx-7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GPX7 aa 1 to C-terminus.

View Alternative Names

GPX6, UNQ469/PRO828, GPX7, Glutathione peroxidase 7, GPx-7, GSHPx-7, CL683

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPx-7 antibody (AB96257)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPx-7 antibody (AB96257)

Immunohistochemical analysis of paraffin-embedded H441, using ab96257 antibody at 1/100 dilution.

Unknown

Western blot - Anti-GPx-7 antibody (AB96257)

12% SDS-PAGE

All lanes:

Western blot - Anti-GPx-7 antibody (ab96257) at 1/500 dilution

Lane 1:

Non transfected 293T cell lysate

Lane 2:

GPx-7 transfected 293T cell lysate

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GPX7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96SL4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glutathione peroxidase 7 (GPx-7) is an enzyme that plays a significant role in protecting cells from oxidative damage. Also known as PHGPx or GPX7 this enzyme belongs to the glutathione peroxidase family. It is involved in reducing hydrogen peroxide and organic hydroperoxides into less reactive molecules using glutathione as a substrate. GPx-7 has a molecular mass of approximately 23 kDa. Expression of GPx-7 is widespread with notable presence in the epithelial cells of the gastrointestinal tract and liver highlighting its potential role in detoxification processes.

Biological function summary

GPx-7 mediates the detoxification of oxidative stress within cells and contributes to maintaining cellular redox balance. It does not act as part of a major protein complex but functions independently to scavenge free radicals and prevent cellular damage. Through its peroxidase activity it helps in preventing the accumulation of harmful peroxides ensuring cellular integrity and stability. This protective mechanism is essential for normal cellular function and survival in oxidative environments.

Pathways

GPx-7 is involved in the glutathione metabolism pathway and the response to oxidative stress pathway. It works closely with other enzymes in the glutathione peroxidase family notably GPx-1 to facilitate the reduction of peroxides using glutathione. These pathways are critical for detoxifying hydrogen peroxide and play an essential role in cellular defense against oxidative damage. GPx-7's cooperation with related family members signifies a coordinated effort in antioxidant defense mechanisms within the cell.

GPx-7 is associated with cancer and neurodegenerative diseases. Abnormal expression and function of GPx-7 can contribute to the progression of these conditions as oxidative stress is a known factor in pathogenesis. In cancer reduced activity or expression of GPx-7 might lead to increased oxidative stress contributing to tumorigenesis. Similarly in neurodegenerative diseases impaired antioxidant defense due to dysfunctional GPx-7 can accelerate neuronal damage. Proteins such as GPx-3 and GPx-8 also relate to these disease mechanisms interacting within similar oxidative pathways further emphasizing the importance of the GPx enzyme family in disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

It protects esophageal epithelia from hydrogen peroxide-induced oxidative stress. It suppresses acidic bile acid-induced reactive oxygen species (ROS) and protects against oxidative DNA damage and double-strand breaks.

See full target information GPX7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 23:131 PubMed37098557

2023

Sodium selenite preserves rBM-MSCs' stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bahareh Rahimi,Mohammad Panahi,Hajie Lotfi,Mostafa Khalili,Astireh Salehi,Neda Saraygord-Afshari,Effat Alizadeh

Nature communications 8:423 PubMed28871080

2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Applications

Unspecified application

Species

Unspecified reactive species

Nai-Jia Huang,Novalia Pishesha,Jean Mukherjee,Sicai Zhang,Rhogerry Deshycka,Valentino Sudaryo,Min Dong,Charles B Shoemaker,Harvey F Lodish

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPx-7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPx-7 antibody (AB96257)

Western blot - Anti-GPx-7 antibody (AB96257)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Primary Antibodies

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