AB183664

Anti-GPX8 antibody

Name: Anti-GPX8 antibody (ab183664) | Abcam
Brand: Abcam Limited
SKU: AB183664
Price: 510 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal GPX8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GPX8 aa 1 to C-terminus.

View Alternative Names

UNQ847/PRO1785, GPX8, Probable glutathione peroxidase 8, GPx-8, GSHPx-8

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPX8 antibody (AB183664)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPX8 antibody (AB183664)

Immunohistochemical analysis of paraffin-embedded X87 xenograft tissue labeling GPX8 with ab183664 at 1/500 dilution.

Supplier Data

Western blot - Anti-GPX8 antibody (AB183664)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-GPX8 antibody (ab183664) at 1/1000 dilution

All lanes:

NT2D1 Whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-GPX8 antibody (AB183664)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-GPX8 antibody (ab183664) at 1/1000 dilution

Lane 1:

Mouse testis tissue extract at 50 µg

Lane 2:

Rat testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GPX8 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TED1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPX8 also known as Glutathione Peroxidase 8 is an enzyme important in cellular antioxidant defense reducing hydrogen peroxide to water. It has a molecular mass of approximately 28 kDa. GPX8 localizes prominently within the endoplasmic reticulum membrane contributing to cellular redox homeostasis. This enzyme expresses in various tissue types with significant levels observed in the liver and kidney indicating its role in detoxifying reactive oxygen species.

Biological function summary

This enzyme plays an important role in maintaining cellular oxidative balance. It does not form part of a large complex but associates with other proteins in a network that mitigates oxidative damage. By catalyzing the reduction of peroxides it protects cellular components from oxidative stress-induced damage. GPX8's activity in reducing lipid peroxides underlines its importance in maintaining cellular membrane integrity particularly under stress conditions.

Pathways

GPX8 integrates into the cellular antioxidant defense system and the redox signaling pathways. It significantly contributes to the Glutathione metabolism pathway partnering with other GPX family members like GPX1. In the context of redox signaling it interacts indirectly with proteins influencing cellular responses to oxidative stress. These interactions facilitate the modulation of signaling cascades important for maintaining cellular homeostasis under oxidative conditions.

GPX8 is associated with oxidative stress-related conditions such as cancer and neurodegenerative diseases. For instance aberrant expression or activity can correlate with increased susceptibility to Hepatocellular Carcinoma where reactive oxygen species play a pivotal role. In neurodegenerative contexts this protein might interact with misfolded protein aggregates potentially implicating pathways linked to Parkinson's disease. As part of this disorder's complex oxidative stress mechanisms GPX8 could interact with proteins like Parkinson-associated DJ-1 highlighting a pathophysiological connection to the disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information GPX8

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular biology 436:168414 PubMed38141874

2023

The Tudor-knot Domain of KAT5 Regulates Nucleosomal Substrate Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Xuan,Hongwen Xuan,Mengying Huang,Wei He,Han Xu,Xiaobing Shi,Hong Wen

International journal of molecular sciences 24: PubMed37958809

2023

mRNA and Protein Expression in Human Fetal Membrane Cells: Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes?

Applications

Unspecified application

Species

Unspecified reactive species

Emmeli Mikkelsen,Berthold Huppertz,Ripudaman Singh,Katarina Ravn,Lotte Hatt,Mogens Kruhøffer,Rheanna Urrabaz-Garza,Niels Uldbjerg,Ramkumar Menon,Torben Steiniche

The European journal of neuroscience 56:5177-5190 PubMed36083288

2022

Astrocytes show increased levels of Ero1α in multiple sclerosis and its experimental autoimmune encephalomyelitis animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Samtleben,Lucas Mina,Megan C Yap,William G Branton,Muhammad Saad Yousuf,Gustavo Tenorio,Klaus Ballanyi,Fabrizio Giuliani,Bradley J Kerr,Christopher Power,Thomas Simmen

Frontiers in genetics 13:898204 PubMed36061208

2022

The overexpression of GPX8 is correlated with poor prognosis in GBM patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sibo Li,Xudong Jiang,Meicun Guan,Yi Zhang,Yanfei Cao,Lina Zhang

BioMed research international 2021:5545312 PubMed34285914

2021

Identification and Validation of Potential Biomarkers and Pathways for Idiopathic Pulmonary Fibrosis by Comprehensive Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Weibin Qian,Xinrui Cai,Qiuhai Qian,Xinying Zhang

Cancer cell international 20:596 PubMed33317536

2020

GPX8 is transcriptionally regulated by FOXC1 and promotes the growth of gastric cancer cells through activating the Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Chen,Lu Xu,Zhi-Li Shan,Shu Chen,Hao Hu

Thoracic cancer 11:3299-3308 PubMed32975378

2020

GPX8 promotes migration and invasion by regulating epithelial characteristics in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Yun Liu,Yan Guo,Qiang Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPX8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPX8 antibody (AB183664)

Western blot - Anti-GPX8 antibody (AB183664)

Western blot - Anti-GPX8 antibody (AB183664)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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Product promise