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Rabbit Recombinant Monoclonal GPX8 antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Rat, Mouse, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-GPX8 antibody [EPR28823-80] (AB317744), expandable thumbnail
  • Western blot - Anti-GPX8 antibody [EPR28823-80] (AB317744), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GPX8 antibody [EPR28823-80] (AB317744), expandable thumbnail
  • Western blot - Anti-GPX8 antibody [EPR28823-80] (AB317744), expandable thumbnail
  • Western blot - Anti-GPX8 antibody [EPR28823-80] (AB317744), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Alternative names

UNQ847/PRO1785, GPX8, Probable glutathione peroxidase 8, GPx-8, GSHPx-8

Recommended products

Rabbit Recombinant Monoclonal GPX8 antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Rat, Mouse, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28823-80
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse ICC

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GPX8 also known as Glutathione Peroxidase 8 is an enzyme important in cellular antioxidant defense reducing hydrogen peroxide to water. It has a molecular mass of approximately 28 kDa. GPX8 localizes prominently within the endoplasmic reticulum membrane contributing to cellular redox homeostasis. This enzyme expresses in various tissue types with significant levels observed in the liver and kidney indicating its role in detoxifying reactive oxygen species.

Biological function summary

This enzyme plays an important role in maintaining cellular oxidative balance. It does not form part of a large complex but associates with other proteins in a network that mitigates oxidative damage. By catalyzing the reduction of peroxides it protects cellular components from oxidative stress-induced damage. GPX8's activity in reducing lipid peroxides underlines its importance in maintaining cellular membrane integrity particularly under stress conditions.

Pathways

GPX8 integrates into the cellular antioxidant defense system and the redox signaling pathways. It significantly contributes to the Glutathione metabolism pathway partnering with other GPX family members like GPX1. In the context of redox signaling it interacts indirectly with proteins influencing cellular responses to oxidative stress. These interactions facilitate the modulation of signaling cascades important for maintaining cellular homeostasis under oxidative conditions.

Associated diseases and disorders

GPX8 is associated with oxidative stress-related conditions such as cancer and neurodegenerative diseases. For instance aberrant expression or activity can correlate with increased susceptibility to Hepatocellular Carcinoma where reactive oxygen species play a pivotal role. In neurodegenerative contexts this protein might interact with misfolded protein aggregates potentially implicating pathways linked to Parkinson's disease. As part of this disorder's complex oxidative stress mechanisms GPX8 could interact with proteins like Parkinson-associated DJ-1 highlighting a pathophysiological connection to the disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744), expandable thumbnail

    Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744)

    This antibody does not cross-react with human GPX7.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: 293T cells transfected with a human GPX7 expression vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: 293T cells transfected with a human GPX8 expression vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution

    Observed band size: 30 kDa, 36 kDa

    Exposure time: 6s

  • Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744), expandable thumbnail

    Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744)

    Negative control: liver

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1: Human uterus tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Human liver tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Rat uterus tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23 kDa, 36 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-GPX8 antibody [EPR28823-80] (ab317744), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPX8 antibody [EPR28823-80] (ab317744)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling GPX8 with ab317744 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing positive staining in Hela cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: HepG2 (PMID: 32817494).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744), expandable thumbnail

    Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744)

    Low expression: Neuro-2a

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: C2C12 (mouse myoblast) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744), expandable thumbnail

    Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744)

    Negative control: HepG2 (PMID: 32817494), Jurkat

    Low expression: MCF7(PMID:32817494)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20 at 20 µg with NFDM/TBST

    Lane 3: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with NFDM/TBST

    Lane 6: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23 kDa, 36 kDa

    Exposure time: 59s

  • Immunoprecipitation - Anti-GPX8 antibody [EPR28823-80] (ab317744), expandable thumbnail

    Immunoprecipitation - Anti-GPX8 antibody [EPR28823-80] (ab317744)

    GPX8 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317744 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317744 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab317744 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317744 in HeLa whole cell lysate

    All lanes: Immunoprecipitation - Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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