Rabbit Polyclonal GRAF antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARHGAP26 aa 600-700.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human ARHGAP26 aa 600-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNA1
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
GTPase-activating protein for RHOA and CDC42.
Alternative names
Rho GTPase-activating protein 26, GTPase regulator associated with focal adhesion kinase, Oligophrenin-1-like protein, Rho-type GTPase-activating protein 26, KIAA0621, OPHN1L, GRAF, ARHGAP26
Recommended products
Rabbit Polyclonal GRAF antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARHGAP26 aa 600-700.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human ARHGAP26 aa 600-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNA1
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226177 was affinity purified using an epitope specific to GRAF immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRAF also known as GAP for Rho-associated domain containing protein F has a molecular mass of approximately 110 kDa. This protein functions mechanically as a GTPase-activating protein (GAP) that regulates Rho family GTPases which are essential in controlling cell shape and movement. GRAF expression is notable in several tissues including the colon brain and heart highlighting its involvement in various physiological processes.
- Biological function summary
GRAF plays an important role in cytoskeletal dynamics impacting cell adhesion and motility. GRAF is part of a larger signaling complex that influences cellular morphology and signal transduction pathways. This interaction underpins its engagement in cellular activities associated with structural changes and response to external stimuli. Through its activity GRAF regulates the balance of actin polymerization and depolymerization essential for maintaining cellular integrity.
- Pathways
GRAF's role intersects with the Rho signaling pathway which is critical for cell mobility and organization. This pathway contains proteins like RhoA and Rac1 where GRAF exerts its GTPase-activating function. GRAF also integrates into the integrin signaling cascade serving as a pivotal link between extracellular matrix interactions and intracellular cytoskeletal arrangements. These pathways establish GRAF's importance in coordinating cellular responses to environmental changes.
- Associated diseases and disorders
GRAF's involvement has been observed in certain cancers particularly colorectal cancer and cardiovascular diseases. In colorectal cancer altered GRAF expression associates with tumor progression and metastasis partly through its interaction with dysregulated RhoA signaling. In cardiovascular conditions changes in GRAF activity influence cardiac myocyte function and may contribute to heart failure. Understanding these connections helps in appreciating GRAF's potential as a therapeutic target in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-GRAF antibody (ab226177)
All lanes: Western blot - Anti-GRAF antibody (ab226177) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 30s
Immunoprecipitation - Anti-GRAF antibody (ab226177)
GRAF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226177 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226177 at 0.4 μg/ml.
Lane 1: ab226177 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-GRAF antibody (ab226177)
Predicted band size: 92 kDa
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