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AB238883

Anti-GRAF antibody

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(1 Publication)

Rabbit Polyclonal GRAF antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP26 aa 600 to C-terminus.

View Alternative Names

GRAF, KIAA0621, OPHN1L, ARHGAP26, Rho GTPase-activating protein 26, GTPase regulator associated with focal adhesion kinase, Oligophrenin-1-like protein, Rho-type GTPase-activating protein 26, GRAF1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (AB238883)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (AB238883)

Paraffin-embedded human colon cancer tissue stained for GRAF with ab238883 at a 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (AB238883)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody (AB238883)

Paraffin-embedded human breast cancer tissue stained for GRAF with ab238883 at a 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARHGAP26 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UNA1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRAF also known as GAP for Rho-associated domain containing protein F has a molecular mass of approximately 110 kDa. This protein functions mechanically as a GTPase-activating protein (GAP) that regulates Rho family GTPases which are essential in controlling cell shape and movement. GRAF expression is notable in several tissues including the colon brain and heart highlighting its involvement in various physiological processes.
Biological function summary

GRAF plays an important role in cytoskeletal dynamics impacting cell adhesion and motility. GRAF is part of a larger signaling complex that influences cellular morphology and signal transduction pathways. This interaction underpins its engagement in cellular activities associated with structural changes and response to external stimuli. Through its activity GRAF regulates the balance of actin polymerization and depolymerization essential for maintaining cellular integrity.

Pathways

GRAF's role intersects with the Rho signaling pathway which is critical for cell mobility and organization. This pathway contains proteins like RhoA and Rac1 where GRAF exerts its GTPase-activating function. GRAF also integrates into the integrin signaling cascade serving as a pivotal link between extracellular matrix interactions and intracellular cytoskeletal arrangements. These pathways establish GRAF's importance in coordinating cellular responses to environmental changes.

GRAF's involvement has been observed in certain cancers particularly colorectal cancer and cardiovascular diseases. In colorectal cancer altered GRAF expression associates with tumor progression and metastasis partly through its interaction with dysregulated RhoA signaling. In cardiovascular conditions changes in GRAF activity influence cardiac myocyte function and may contribute to heart failure. Understanding these connections helps in appreciating GRAF's potential as a therapeutic target in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein for RHOA and CDC42. Facilitates mitochondrial quality control by promoting Parkin-mediated recruitment of autophagosomes to damaged mitochondria (PubMed : 38081847). Negatively regulates the growth of human parainfluenza virus type 2 by inhibiting hPIV-2-mediated RHOA activation via interaction with two of its viral proteins P and V (PubMed : 27512058).. Isoform 2. Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation of MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins.
See full target information ARHGAP26
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:105358 PubMed36339256

2022

Cellular plasticity and immune microenvironment of malignant pleural effusion are associated with EGFR-TKI resistance in non-small-cell lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyoung-Oh Jeong,Hayoon Lee,Hyemin Kim,Jinho Jang,Seunghoon Kim,Taejoo Hwang,David Whee-Young Choi,Hong Sook Kim,Naeun Lee,Yoo Mi Lee,Sehhoon Park,Hyun Ae Jung,Jong-Mu Sun,Jin Seok Ahn,Myung-Ju Ahn,Keunchil Park,Semin Lee,Se-Hoon Lee
View all publications
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Product promise

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