AB137085

Anti-GRAF antibody [EPR8252]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal GRAF antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

GRAF, KIAA0621, OPHN1L, ARHGAP26, Rho GTPase-activating protein 26, GTPase regulator associated with focal adhesion kinase, Oligophrenin-1-like protein, Rho-type GTPase-activating protein 26, GRAF1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody [EPR8252] (AB137085)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody [EPR8252] (AB137085)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling GRAF with ab137085 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-GRAF antibody [EPR8252] (AB137085)

All lanes:

Western blot - Anti-GRAF antibody [EPR8252] (ab137085) at 1/1000 dilution

Lane 1:

Human fetal heart lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

MCF7 cell lysates at 10 µg

Lane 4:

Human fetal brain lysates at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8252

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRAF also known as GAP for Rho-associated domain containing protein F has a molecular mass of approximately 110 kDa. This protein functions mechanically as a GTPase-activating protein (GAP) that regulates Rho family GTPases which are essential in controlling cell shape and movement. GRAF expression is notable in several tissues including the colon brain and heart highlighting its involvement in various physiological processes.

Biological function summary

GRAF plays an important role in cytoskeletal dynamics impacting cell adhesion and motility. GRAF is part of a larger signaling complex that influences cellular morphology and signal transduction pathways. This interaction underpins its engagement in cellular activities associated with structural changes and response to external stimuli. Through its activity GRAF regulates the balance of actin polymerization and depolymerization essential for maintaining cellular integrity.

Pathways

GRAF's role intersects with the Rho signaling pathway which is critical for cell mobility and organization. This pathway contains proteins like RhoA and Rac1 where GRAF exerts its GTPase-activating function. GRAF also integrates into the integrin signaling cascade serving as a pivotal link between extracellular matrix interactions and intracellular cytoskeletal arrangements. These pathways establish GRAF's importance in coordinating cellular responses to environmental changes.

GRAF's involvement has been observed in certain cancers particularly colorectal cancer and cardiovascular diseases. In colorectal cancer altered GRAF expression associates with tumor progression and metastasis partly through its interaction with dysregulated RhoA signaling. In cardiovascular conditions changes in GRAF activity influence cardiac myocyte function and may contribute to heart failure. Understanding these connections helps in appreciating GRAF's potential as a therapeutic target in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase-activating protein for RHOA and CDC42. Facilitates mitochondrial quality control by promoting Parkin-mediated recruitment of autophagosomes to damaged mitochondria (PubMed : 38081847). Negatively regulates the growth of human parainfluenza virus type 2 by inhibiting hPIV-2-mediated RHOA activation via interaction with two of its viral proteins P and V (PubMed : 27512058).. Isoform 2. Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation of MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins.

See full target information ARHGAP26

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal 39:1910-1929 PubMed30626696

2019

Pyk2 Signaling through Graf1 and RhoA GTPase Is Required for Amyloid-β Oligomer-Triggered Synapse Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Suho Lee,Santiago V Salazar,Timothy O Cox,Stephen M Strittmatter

IJU case reports 2:43-46 PubMed32743370

2018

Successful treatment with sorafenib for sunitinib-refractory metastatic papillary renal cell carcinoma: Potential impact of Raf overexpression on predicting the efficacy of sorafenib.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Morinaga,Takashi Kawahara,Jun-Ichi Teranishi,Makoto Chuma,Koji Izumi,Yasuhide Miyoshi,Masahiro Yao,Masako Otani,Hiroshi Miyamoto,Hiroji Uemura

The Journal of international medical research 46:5019-5029 PubMed30387365

2018

RhoA/ROCK/ARHGAP26 signaling in the eutopic and ectopic endometrium is involved in clinical characteristics of adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Jiang,Wei Gong,Rong Chen,Huihui Ke,Xiaoyan Qu,Weihong Yang,Zhongping Cheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRAF antibody [EPR8252]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody [EPR8252] (AB137085)

Western blot - Anti-GRAF antibody [EPR8252] (AB137085)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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