Rabbit Polyclonal Grainyhead-like protein 1 homolog antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL1 aa 1-250.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GRHL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZI5
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Target data
Function
Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:12175488, PubMed:18288204, PubMed:29309642). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity).Isoform 1Functions as transcription activator.Isoform 2May function as a repressor in tissues where both isoform 1 and isoform 2 are expressed.
Alternative names
Grainyhead-like protein 1 homolog, Mammalian grainyhead, NH32, Transcription factor CP2-like 2, Transcription factor LBP-32, TFCP2L2, GRHL1, LBP32, MGR
Recommended products
Rabbit Polyclonal Grainyhead-like protein 1 homolog antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL1 aa 1-250.
Alternative names
Grainyhead-like protein 1 homolog, Mammalian grainyhead, NH32, Transcription factor CP2-like 2, Transcription factor LBP-32, TFCP2L2, GRHL1, LBP32, MGR
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GRHL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NZI5
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab111582 is purified by antigen affinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Grainyhead-like protein 1 homolog known as GRHL1 plays an important role in transcription regulation. It functions as a transcription factor binding to DNA and influencing gene expression. This protein has a molecular mass of approximately 74 kDa and is expressed in various tissues with a strong presence in the skin and other epithelial tissues. GRHL1 is important for maintaining the integrity of epithelial cells which form protective barriers in the body.
- Biological function summary
GRHL1 controls processes related to cell differentiation adhesion and proliferation. It does not function in isolation but rather forms part of transcriptional complexes that modify the expression of target genes. By doing so GRHL1 helps regulate the development and maintenance of epithelial structures. Its activity supports the transcription of genes necessary for the formation of cell junctions and maintenance of epithelial morphogenesis.
- Pathways
GRHL1 integrates into several key biological processes notably the Wnt signaling pathway and cell adhesion pathways. In the Wnt pathway GRHL1 interacts with known signaling regulators like β-catenin to influence cell fate decisions and tissue homeostasis. GRHL1's role in cell adhesion pathways helps modulate interactions between epithelial cells working with proteins such as E-cadherin to ensure proper adhesive functions and cellular organization.
- Associated diseases and disorders
GRHL1 has associations with skin conditions and some types of cancer. Altered expression or mutations in GRHL1 and its pathways can contribute to disorders like ectodermal dysplasia a condition affecting the development of skin hair and nails. Additionally changes in GRHL1 levels and its activity have links to tumorigenesis in epithelial tissues often in conjunction with disrupted E-cadherin functions. This makes it a potential marker or therapeutic target in certain epithelial-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Grainyhead-like protein 1 homolog antibody (ab111582)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-Grainyhead-like protein 1 homolog antibody (ab111582) at 1/1000 dilution
All lanes: NT2D1 whole cell lysate at 30 µg
Predicted band size: 70 kDa, 76 kDa, 89 kDa, 92 kDa, 93 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Grainyhead-like protein 1 homolog antibody (ab111582)
ab111582 at 1/500 dilution staining Grainyhead-like protein 1 homolog in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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