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AB111582

Anti-Grainyhead-like protein 1 homolog antibody

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(2 Publications)

Rabbit Polyclonal Grainyhead-like protein 1 homolog antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRHL1 aa 1-250.

View Alternative Names

LBP32, MGR, TFCP2L2, GRHL1, Grainyhead-like protein 1 homolog, Mammalian grainyhead, NH32, Transcription factor CP2-like 2, Transcription factor LBP-32

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Grainyhead-like protein 1 homolog antibody (AB111582)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Grainyhead-like protein 1 homolog antibody (AB111582)

ab111582 at 1/500 dilution staining Grainyhead-like protein 1 homolog in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-Grainyhead-like protein 1 homolog antibody (AB111582)
  • WB

Unknown

Western blot - Anti-Grainyhead-like protein 1 homolog antibody (AB111582)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Grainyhead-like protein 1 homolog antibody (ab111582) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 70 kDa,76 kDa,89 kDa,92 kDa,93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRHL1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NZI5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111582 is purified by antigen affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Grainyhead-like protein 1 homolog known as GRHL1 plays an important role in transcription regulation. It functions as a transcription factor binding to DNA and influencing gene expression. This protein has a molecular mass of approximately 74 kDa and is expressed in various tissues with a strong presence in the skin and other epithelial tissues. GRHL1 is important for maintaining the integrity of epithelial cells which form protective barriers in the body.
Biological function summary

GRHL1 controls processes related to cell differentiation adhesion and proliferation. It does not function in isolation but rather forms part of transcriptional complexes that modify the expression of target genes. By doing so GRHL1 helps regulate the development and maintenance of epithelial structures. Its activity supports the transcription of genes necessary for the formation of cell junctions and maintenance of epithelial morphogenesis.

Pathways

GRHL1 integrates into several key biological processes notably the Wnt signaling pathway and cell adhesion pathways. In the Wnt pathway GRHL1 interacts with known signaling regulators like β-catenin to influence cell fate decisions and tissue homeostasis. GRHL1's role in cell adhesion pathways helps modulate interactions between epithelial cells working with proteins such as E-cadherin to ensure proper adhesive functions and cellular organization.

GRHL1 has associations with skin conditions and some types of cancer. Altered expression or mutations in GRHL1 and its pathways can contribute to disorders like ectodermal dysplasia a condition affecting the development of skin hair and nails. Additionally changes in GRHL1 levels and its activity have links to tumorigenesis in epithelial tissues often in conjunction with disrupted E-cadherin functions. This makes it a potential marker or therapeutic target in certain epithelial-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed : 12175488, PubMed : 18288204, PubMed : 29309642). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, nor functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity). May play a role in regulating glucose homeostasis and insulin signaling.. Isoform 1. Functions as a transcription activator.. Isoform 2. May function as a repressor in tissues where both isoform 1 and isoform 2 are expressed.
See full target information GRHL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7524 PubMed37980342

2023

The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav Saha,Shar-Yin Naomi Huang,Xi Yang,Liton Kumar Saha,Yilun Sun,Prashant Khandagale,Lisa M Jenkins,Yves Pommier

EMBO molecular medicine 15:e17836 PubMed37766669

2023

Propionate reinforces epithelial identity and reduces aggressiveness of lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh Ramesh,Paradesi Naidu Gollavilli,Luisa Pinna,Mohammad Aarif Siddiqui,Adriana Martinez Turtos,Francesca Napoli,Yasmin Antonelli,Aldo Leal-Egaña,Jesper Foged Havelund,Simon Toftholm Jakobsen,Elisa Le Boiteux,Marco Volante,Nils Joakim Faergeman,Ole N Jensen,Rasmus Siersbaek,Kumar Somyajit,Paolo Ceppi
View all publications
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Product promise

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