JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267414

Anti-Gram Positive Bacteria LTA antibody [G43J]

Be the first to review this product! Submit a review

|

(5 Publications)

Mouse Monoclonal Gram Positive Bacteria antibody. Suitable for WB and reacts with Bacillus subtilis, Gram Positive Bacteria samples. Cited in 5 publications.
1 Images
Western blot - Anti-Gram Positive Bacteria LTA antibody [G43J] (AB267414)
  • WB

Supplier Data

Western blot - Anti-Gram Positive Bacteria LTA antibody [G43J] (AB267414)

Wild-type B. subtilis or a mutant form of B. subtilis lacking LTA were grown to the same optical density and lysed.

All lanes:

Western blot - Anti-Gram Positive Bacteria LTA antibody [G43J] (ab267414) at 1/250 dilution

Lane 1:

Wild-type B. subtilis LTA at 65 µg

Lane 2:

Mutant form of B. subtilis lacking LTA at 65 µg

Secondary

All lanes:

Alkaline phosphatase conjugated secondary antibody and NBT/BCIPsubstrate.

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

G43J

Isotype

IgG1

Carrier free

No

Reacts with

Bacillus subtilis, Gram Positive Bacteria

Applications

WB

applications

Specificity

THis antibody reacts with Gram positive bacteria Lipoteichoic acid (LTA) in bacterial and infected tissue samples.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacillus subtilis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10 - 1/250", "WB-species-notes": "<p></p>" }, "Gram Positive Bacteria": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10 - 1/250", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The term 'Gram-positive bacteria' refers to a group of bacteria that retain the crystal violet stain during the Gram stain procedure. This staining technique distinguishes them from Gram-negative bacteria by their thicker peptidoglycan layer. Common examples of Gram-positive bacteria include Bacillus subtilis which is often used as a model organism in research. Gram-positive bacteria are characterized by a thicker cell wall that contains teichoic acids contributing to a density that assists in stain retention. This structural distinction enables them to be easily identified under a microscope during laboratory diagnostics and microbial analyses.
Biological function summary

Gram-positive bacteria exhibit diverse functions and roles within ecosystems and human health. They play significant roles in symbiotic relationships and nutrient cycling as well as act as pathogens in various infections. They can form biofilms and can sporulate enhancing their survival in harsh environments. Gram-positive bacteria are not part of a specific complex but they engage in diverse interactions with other microorganisms and host tissues impacting biological processes such as colonization and infection.

Pathways

Gram-positive bacteria participate in numerous important biological and metabolic pathways. For instance they are involved in the glycolytic pathway and the peptidoglycan synthesis pathway important for maintaining their cell structure and energy metabolism. Proteins like penicillin-binding proteins play an important role in the synthesis and modification of peptidoglycan. Similarly autolysins are involved in cell wall remodeling and turnover which are important for cell growth and division.

Gram-positive bacteria are connected to several infections including skin infections and pneumonia. Staphylococcus aureus a well-known Gram-positive bacterium can lead to conditions ranging from minor skin infections to severe diseases like sepsis. These bacteria interact with host immune proteins such as cytokines often resulting in inflammatory responses. Additionally Streptococcus pneumoniae another Gram-positive bacterium is associated with pneumonia and meningitis illustrating their relevance in public health concerns.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:1278 PubMed40775324

2025

Evaluating IL22RA1 expression as a predictive indicator in human colon cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yin,Renhao Geng,Junjun Chen,Bin Xu,Hui Ma,Yingting Liu,Xiao Zheng,Lujun Chen

Scientific reports 15:27263 PubMed40715596

2025

Comparative and pharmacological investigation of bEVs from eight Lactobacillales strains.

Applications

Unspecified application

Species

Unspecified reactive species

Seoah Park,Jongsoo Mok,Hye-Min Yu,Hye-Jin An,Ga-Hyun Choi,Yeon-Seon Lee,Ki-Jin Kwon,Sung-Jun Choi,Kyung-Hee Kim,Soo-Jin Kim,Joonghoon Park

International journal of molecular sciences 26: PubMed39940832

2025

A Simplified Method for the Isolation of Extracellular Vesicles from Probiotic Bacteria and Their Characterization.

Applications

Unspecified application

Species

Unspecified reactive species

Harshal Sawant,Ji Bihl,Alip Borthakur

Materials (Basel, Switzerland) 15: PubMed36363232

2022

Direct, Rapid Detection of Pathogens from Urine Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Sorin David,Raluca-Elena Munteanu,Ana-Maria Tițoiu,Ionela-Cristina Petcu,Ioana-Cristina Cernat,Corina Leancu,Mihaela Gheorghiu,Eugen Gheorghiu

Frontiers in cellular and infection microbiology 11:712360 PubMed34604106

2021

An Mixed Infection Model With Commensal and Pathogenic Staphylococci for the Exploration of Interspecific Interactions and Their Impacts on Skin Physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Katsunori Kohda,Xuan Li,Naoki Soga,Risa Nagura,Tie Duerna,Saeko Nakajima,Ichiro Nakagawa,Masakazu Ito,Akinori Ikeuchi
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com