Rabbit Polyclonal GRAMD1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRAMD1B aa 500-650.
Images
Publications
Filter by
- Scientific reports 8:95112018GRAMD1B regulates cell migration in breast cancer cells through JAK/STAT and Akt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPuja Khanna,Joan Shuying Lee,Amornpun Sereemaspun,Haeryun Lee,Gyeong Hun BaegPubMed 29934528
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GRAMD1B aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q3KR37
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity).
Alternative names
KIAA1201, UNQ3032/PRO9834, GRAMD1B, Protein Aster-B, GRAM domain-containing protein 1B
Recommended products
Rabbit Polyclonal GRAMD1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRAMD1B aa 500-650.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GRAMD1B aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q3KR37
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRAMD1B also called Aster-B is a phospholipid transporter with a mass of approximately 89 kDa. It plays a significant role in cholesterol regulation by transferring cholesterol from the plasma membrane to the endoplasmic reticulum. GRAMD1B is expressed in various tissues including the liver and brain. Its activity depends on the binding to phosphatidylserine-rich domains within the plasma membrane. This protein also contains a GRAM domain which aids in its lipid-binding properties and interaction with membranes enriched in specific lipids.
- Biological function summary
This protein assists in maintaining lipid homeostasis by facilitating cholesterol transport. GRAMD1B performs its function as part of a larger protein complex that includes GRAMD1A and GRAMD1C. This association suggests a cooperative mechanism in regulating lipid distribution and dynamics within the cell. Through this complex GRAMD1B contributes to membrane biogenesis and possibly plays a role in cellular signaling pathways that rely on cholesterol and specific phospholipids.
- Pathways
GRAMD1B is involved in the phosphoinositide signaling pathway where its lipid-binding properties are essential. The protein interacts with other important signaling molecules like AKT and PI3K influencing downstream signaling cascades that affect cellular growth and metabolism. Additionally GRAMD1B might play a role in the sphingolipid metabolism pathway where its actions could alter the composition of lipid microdomains within the cell membrane and affect signal transduction.
- Associated diseases and disorders
GRAMD1B has been linked to metabolic disorders such as non-alcoholic fatty liver disease (NAFLD). Research suggests that dysregulation of cholesterol transport could contribute to the development of NAFLD. Furthermore GRAMD1B shows a connection to neurodegenerative diseases through interactions with proteins like ApoE which plays a role in lipid transport and is associated with Alzheimer's disease. This connection suggests that alterations in lipid homeostasis due to GRAMD1B dysfunction may contribute to the pathophysiology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1B antibody (ab121286)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab121286 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1B antibody (ab121286)
ab121286, at a 1/100 dilution, staining GRAMD1B in paraffin-embedded Human gall bladder tissue by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com