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AB121286

Anti-GRAMD1B antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal GRAMD1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRAMD1B aa 500-650.

View Alternative Names

KIAA1201, UNQ3032/PRO9834, GRAMD1B, Protein Aster-B, GRAM domain-containing protein 1B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1B antibody (AB121286)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1B antibody (AB121286)

ab121286, at a 1/100 dilution, staining GRAMD1B in paraffin-embedded Human gall bladder tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1B antibody (AB121286)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1B antibody (AB121286)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab121286 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GRAMD1B aa 500-650. The exact immunogen used to generate this antibody is proprietary information.

Q3KR37

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRAMD1B also called Aster-B is a phospholipid transporter with a mass of approximately 89 kDa. It plays a significant role in cholesterol regulation by transferring cholesterol from the plasma membrane to the endoplasmic reticulum. GRAMD1B is expressed in various tissues including the liver and brain. Its activity depends on the binding to phosphatidylserine-rich domains within the plasma membrane. This protein also contains a GRAM domain which aids in its lipid-binding properties and interaction with membranes enriched in specific lipids.
Biological function summary

This protein assists in maintaining lipid homeostasis by facilitating cholesterol transport. GRAMD1B performs its function as part of a larger protein complex that includes GRAMD1A and GRAMD1C. This association suggests a cooperative mechanism in regulating lipid distribution and dynamics within the cell. Through this complex GRAMD1B contributes to membrane biogenesis and possibly plays a role in cellular signaling pathways that rely on cholesterol and specific phospholipids.

Pathways

GRAMD1B is involved in the phosphoinositide signaling pathway where its lipid-binding properties are essential. The protein interacts with other important signaling molecules like AKT and PI3K influencing downstream signaling cascades that affect cellular growth and metabolism. Additionally GRAMD1B might play a role in the sphingolipid metabolism pathway where its actions could alter the composition of lipid microdomains within the cell membrane and affect signal transduction.

GRAMD1B has been linked to metabolic disorders such as non-alcoholic fatty liver disease (NAFLD). Research suggests that dysregulation of cholesterol transport could contribute to the development of NAFLD. Furthermore GRAMD1B shows a connection to neurodegenerative diseases through interactions with proteins like ApoE which plays a role in lipid transport and is associated with Alzheimer's disease. This connection suggests that alterations in lipid homeostasis due to GRAMD1B dysfunction may contribute to the pathophysiology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity).
See full target information GRAMD1B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:9511 PubMed29934528

2018

GRAMD1B regulates cell migration in breast cancer cells through JAK/STAT and Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Puja Khanna,Joan Shuying Lee,Amornpun Sereemaspun,Haeryun Lee,Gyeong Hun Baeg
View all publications
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Product promise

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