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AB121365

Anti-GRAMD1C antibody

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(1 Publication)

Rabbit Polyclonal GRAMD1C antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRAMD1C aa 450-550.

View Alternative Names

UNQ2543/PRO6095, GRAMD1C, Protein Aster-C, GRAM domain-containing protein 1C

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1C antibody (AB121365)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD1C antibody (AB121365)

ab121365 at 1/500 dilution staining GRAMD1C in Paraffin-emebedded Human Smooth muscle tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1C antibody (AB121365)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRAMD1C antibody (AB121365)

Immunofluorescent staining of Human cell line U-251MG shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121365 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-GRAMD1C antibody (AB121365)
  • WB

Unknown

Western blot - Anti-GRAMD1C antibody (AB121365)

All lanes:

Western blot - Anti-GRAMD1C antibody (ab121365) at 1/250 dilution

Lane 1:

RT4 lysate

Lane 2:

U251 MG lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 76 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GRAMD1C aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q8IYS0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRAM domain-containing 1C (GRAMD1C) is a protein involved in the mechanical process of cell organization and regulation. It is also known as Aster-C. GRAMD1C has a molecular weight of approximately 57 kDa. This protein is expressed in various tissues but shows higher expression levels in liver and testis. GRAMD1C contains a GRAM domain and a VASt domain that contribute to its role in lipid transfer and membrane binding processes.
Biological function summary

GRAMD1C plays a role in the transport and metabolism of sterols and phosphatidylserine. It does not function alone but is part of a larger complex that includes additional family members like GRAMD1A and GRAMD1B. By coordinating with these proteins GRAMD1C helps maintain lipid homeostasis in cells. These functions are essential for normal cellular processes and contribute to membrane dynamics and signaling.

Pathways

GRAMD1C impacts the cholesterol homeostasis pathway and the phosphatidylserine translocation pathway. In the cholesterol homeostasis pathway GRAMD1C works with other related proteins such as STARD4 to facilitate sterol transport within the cell. The phosphatidylserine translocation pathway benefits from GRAMD1C's involvement by positioning lipids correctly on the cell membrane helping to ensure proper signaling and cell survival functions.

GRAMD1C shows a relationship with metabolic disorders and liver-related diseases. Altered function or expression of GRAMD1C may contribute to conditions such as non-alcoholic fatty liver disease. Its connection with other proteins like GRAMD1A in lipid metabolism suggests that disruptions within this family could lead to metabolic syndrome. Understanding these relations helps identify potential therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity).
See full target information GRAMD1C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2414723 PubMed39960327

2025

Fam172a Mediates the Stimulation of Hypothalamic Oxytocin Neurons to Suppress Obesity-Induced Anxiety.

Applications

Unspecified application

Species

Unspecified reactive species

Baocheng Wan,Lina Zhang,Xinyu Wang,Rong Zhang,Lianxi Li,Yi Zhang,Zhuo Chen,Cheng Hu
View all publications
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