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AB154861

Anti-GRAMD2 antibody [EPR10060]

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal GRAMD2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GRAMD2, GRAMD2A, GRAM domain-containing protein 2A

1 Images
Western blot - Anti-GRAMD2 antibody [EPR10060] (AB154861)
  • WB

Unknown

Western blot - Anti-GRAMD2 antibody [EPR10060] (AB154861)

All lanes:

Western blot - Anti-GRAMD2 antibody [EPR10060] (ab154861) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10060

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRAMD2 also known as GRAM domain containing 2 is a protein with a molecular mass of approximately 67 kDa. It exists within various cellular compartments including the endoplasmic reticulum (ER) and the Golgi apparatus. Its expression is generally widespread but it is particularly noticeable in the brain and several other tissues. Mechanically GRAMD2 participates in membrane interactions and lipids binding which helps maintain structural organization within cells. The GRAM domain within GRAMD2 is significant for these interactions allowing the protein to bind to specific lipid membranes.
Biological function summary

The GRAM domain along with additional structural features of GRAMD2 plays a role in lipid-related cellular processes. GRAMD2 acts as a part of larger protein complexes that are responsible for regulating intracellular signaling and trafficking. Its interactions with lipid membranes are essential for maintaining cellular homeostasis particularly involving lipid metabolism. This coordination is essential for various cellular functions including the response to cellular stress and membrane repair mechanisms.

Pathways

GRAMD2 integrates into cellular signaling and lipid metabolism pathways. It is notably linked with the mTOR signaling pathway an important regulator of cell growth and homeostasis. GRAMD2 interacts with proteins like mTOR and Rheb which are involved in energy sensing and metabolic regulation. Another connected pathway is the phosphoinositide signaling pathway which further involves GRAMD2 in regulating lipid dynamics within the cell emphasizing its role in sustaining cellular balance.

GRAMD2 alterations connect to conditions like neurodegenerative diseases and specific forms of cancer. It shows associations with proteins such as Tau in Alzheimer's disease suggesting that changes in lipid binding and processing could affect tau pathology. Additionally dysregulation of GRAMD2 has been implicated in lipid metabolism disorders a factor contributing to metabolic syndromes. These connections highlight the importance of GRAMD2 in maintaining lipid balance influencing disease progression and highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the organization of endoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).
See full target information GRAMD2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5393-5410 PubMed40959270

2025

Altered Heme and Redox Homeostasis Underpin Late-onset Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Adedamola Saidi Soladogun,Chantal Vidal,Maria Del Carmen Chacon Castro,Heng Du,Li Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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