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AB234649

Anti-GRAMD4 antibody

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(1 Publication)

Rabbit Polyclonal GRAMD4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRAMD4 aa 1-200.

View Alternative Names

DIP, KIAA0767, GRAMD4, GRAM domain-containing protein 4, Death-inducing protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD4 antibody (AB234649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAMD4 antibody (AB234649)

Paraffin-embedded human breast cancer tissue stained for GRAMD4 using ab234649 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-GRAMD4 antibody (AB234649)
  • WB

Supplier Data

Western blot - Anti-GRAMD4 antibody (AB234649)

All lanes:

Western blot - Anti-GRAMD4 antibody (ab234649) at 1/500 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 66 kDa

false

Western blot - Anti-GRAMD4 antibody (AB234649)
  • WB

Supplier Data

Western blot - Anti-GRAMD4 antibody (AB234649)

All lanes:

Western blot - Anti-GRAMD4 antibody (ab234649) at 1/500 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRAMD4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q6IC98

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRAMD4 also known as Genes Related to Apoptosis and Mitochondria 4 is involved mechanically in the regulation of apoptosis. It governs cellular processes through its influence on mitochondrial membrane permeability. The protein has a mass of approximately 44 kDa. GRAMD4 is expressed in several tissues with high presence noted in the brain and heart tissues. It is a part of the signaling mechanisms that manage programmed cell death impacting cellular health significantly.
Biological function summary

GRAMD4 interacts with specific partners in apoptosis-related pathways. It aids in regulating the balance between cell survival and death by modulating mitochondrial activities. It participates as a component of molecular complexes that trigger apoptotic signaling cascades promoting the release of apoptogenic factors. Through these interactions GRAMD4 maintains cellular equilibrium and ensures proper cellular function by overseeing cellular responses to stress.

Pathways

The role of GRAMD4 becomes evident in the intrinsic apoptosis pathway. GRAMD4 collaborates with BCL2 family proteins in managing mitochondrial pathways that lead to apoptosis. It facilitates the communication between apoptotic signals and mitochondrial responses. Additionally GRAMD4 functions in pathways associated with cellular stress responses alongside proteins like P53 that further mediate these pathways. These interactions place GRAMD4 as an integral component in cellular homeostasis and stress management.

GRAMD4 has connections to neurodegenerative diseases and cancer. Dysregulation of this protein can contribute to improper apoptosis which features prominently in conditions like Parkinson's disease and various types of cancer. In these contexts GRAMD4 influences cellular outcomes by interacting with proteins such as BAX and P53 further linking it to disease progression. Its role in apoptosis makes GRAMD4 a significant focus of research in understanding and potentially mitigating disease effects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53 (PubMed : 15565177). Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53. Inhibits TLR9 response to nucelic acids and regulates TLR9-mediated innate immune response (By similarity).
See full target information GRAMD4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e23577 PubMed37934488

2023

Silencing of GDF11 suppresses hepatocyte apoptosis to relieve LPS/D-GalN acute liver failure.

Applications

Unspecified application

Species

Unspecified reactive species

Rongsheng Zhou,Shuang Li,Qun Wang,Yang Bi,Xiaogang Li,Qiang Wang
View all publications
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