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AB231632

Anti-Grancalcin antibody

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(1 Publication)

Rabbit Polyclonal Grancalcin antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GCA aa 1-150.

View Alternative Names

GCL, GCA, Grancalcin

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)

Paraffin-embedded human liver tissue stained for Grancalcin using ab231632 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)

Paraffin-embedded human stomach tissue stained for Grancalcin using ab231632 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)

Paraffin-embedded human prostate cancer tissue stained for Grancalcin using ab231632 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)

Paraffin-embedded human kidney tissue stained for Grancalcin using ab231632 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)

Paraffin-embedded human cerebrum tissue stained for Grancalcin using ab231632 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grancalcin antibody (AB231632)

Paraffin-embedded human glioma tissue stained for Grancalcin using ab231632 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Grancalcin antibody (AB231632)
  • WB

Supplier Data

Western blot - Anti-Grancalcin antibody (AB231632)

All lanes:

Western blot - Anti-Grancalcin antibody (ab231632) at 1 µg/mL

Lane 1:

Human serum

Lane 2:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Lane 3:

Sp2/0 cell lysate

Lane 4:

Rat spleen lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 24 kDa

false

Western blot - Anti-Grancalcin antibody (AB231632)
  • WB

Supplier Data

Western blot - Anti-Grancalcin antibody (AB231632)

All lanes:

Western blot - Anti-Grancalcin antibody (ab231632) at 1 µg/mL

All lanes:

Recombinant human Grancalcin protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GCA aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P28676

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231632 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Grancalcin also known as GCL or GCA is a calcium-binding protein that belongs to the penta-EF-hand family. The protein has a molecular mass of about 24 kDa. It is predominantly found in neutrophils and monocytes where it localizes to the cytosol and cell membrane upon calcium activation. Grancalcin is known for its role in calcium signaling as its function is calcium-dependent which aids in modulating cellular processes.
Biological function summary

Grancalcin is involved in the regulation of cellular adhesion mechanisms. It does not form part of a larger protein complex but it plays a critical role in integrating calcium signals that facilitate cell function. Additionally grancalcin helps in the reorganization of the cytoskeleton impacting processes like chemotaxis and phagocytosis important for immune response.

Pathways

Grancalcin plays a role in the integrin signaling pathway. It interacts with key proteins in this pathway such as talin and vinculin to mediate cell adhesion and motility. Grancalcin's regulation of calcium signals also ties it to the MAPK signaling pathway which influences various cell functions including proliferation and differentiation.

Alterations in grancalcin expression or function have connections to immune-related diseases such as chronic inflammation. Aberrant levels may also have implications in cancer metastasis where cell adhesion and motility affect tumor spread. Grancalcin's role in these conditions may involve interactions with integrin-associated proteins that modulate the mechanical properties of cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.
See full target information GCA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 15:e1008378 PubMed31479451

2019

NME5 frameshift variant in Alaskan Malamutes with primary ciliary dyskinesia.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Anderegg,Michelle Im Hof Gut,Udo Hetzel,Elizabeth W Howerth,Fabienne Leuthard,Kaisa Kyöstilä,Hannes Lohi,Louise Pettitt,Cathryn Mellersh,Katie M Minor,James R Mickelson,Kevin Batcher,Danika Bannasch,Vidhya Jagannathan,Tosso Leeb
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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