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AB191211

Anti-Progranulin antibody

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(8 Publications)

Rabbit Polyclonal Granulin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRN aa 1-350.

View Alternative Names

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN

1 Images
Western blot - Anti-Progranulin antibody (AB191211)
  • WB

Supplier Data

Western blot - Anti-Progranulin antibody (AB191211)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Progranulin antibody (ab191211) at 1/1000 dilution

Lane 1:

HL60 cell line lysate at 25 µg

Lane 2:

THP1 cell line lysate at 25 µg

Lane 3:

PC12 cell line lysate at 25 µg

Lane 4:

293T cell line lysate at 25 µg

Lane 5:

HepG2 cell line lysate at 25 µg

Lane 6:

MCF7 cell line lysate at 25 µg

Lane 7:

Mouse liver tissue lysate at 25 µg

Lane 8:

Mouse spleen tissue lysate at 25 µg

Lane 9:

Mouse kidney tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 64 kDa

true

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human GRN aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P28799

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12526812, PubMed : 18378771, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286). Regulates protein trafficking to lysosomes, and also the activity of lysosomal enzymes (PubMed : 28453791, PubMed : 28541286). Also facilitates the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 12526812). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 18378771).. Granulin-4. Promotes proliferation of the epithelial cell line A431 in culture.. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
See full target information GRN
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 29:33 PubMed40486898

2025

Effect of overexpression of on the proliferation and osteogenic capacity of human periodontal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanxuan Yao,Ruoshan Qin,Ziwei Cui,Dengqi He,Xiaorong Sun,Yuefeng Sun,Xiangyi He

The Journal of clinical investigation 134: PubMed39264847

2024

Lactate supports Treg function and immune balance via MGAT1 effects on N-glycosylation in the mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Jinren Zhou,Jian Gu,Qufei Qian,Yigang Zhang,Tianning Huang,Xiangyu Li,Zhuoqun Liu,Qing Shao,Yuan Liang,Lei Qiao,Xiaozhang Xu,Qiuyang Chen,Zibo Xu,Yu Li,Ji Gao,Yufeng Pan,Yiming Wang,Roderick O'Connor,Keli L Hippen,Ling Lu,Bruce R Blazar

Physiological research 72:475-483 PubMed37795890

2023

Matrine From Sophora Flavescens Attenuates on Collagen-Induced Osteoarthritis by Modulating the Activity of miR-29B-3P/PGRN Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Q Jin,Z Li,Q Xu,Q Liu

PLoS pathogens 15:e1008062 PubMed31585000

2019

Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fanhua Wei,Zhimin Jiang,Honglei Sun,Juan Pu,Yipeng Sun,Mingyang Wang,Qi Tong,Yuhai Bi,Xiaojing Ma,George Fu Gao,Jinhua Liu

Cancer letters 465:24-35 PubMed31491449

2019

Progranulin promotes melanoma progression by inhibiting natural killer cell recruitment to the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Ramouna Voshtani,Mei Song,Huan Wang,Xiaoqi Li,Wei Zhang,Mojdeh S Tavallaie,Wenjun Yan,Joseph Sun,Fang Wei,Xiaojing Ma

Journal of cellular physiology 233:6615-6631 PubMed29150939

2018

miR-34b-5p inhibition attenuates lung inflammation and apoptosis in an LPS-induced acute lung injury mouse model by targeting progranulin.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Xie,Qingchun Lu,Kailing Wang,Jingjing Lu,Xia Gu,Dongyi Zhu,Fanglei Liu,Zhongliang Guo

Evidence-based complementary and alternative medic 2018:5738101 PubMed29743924

2018

Emodin Alleviates Liver Fibrosis of Mice by Reducing Infiltration of Gr1 Monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-An Zhao,Guangmei Chen,Yong Liu,Hongyan Wu,Jin Chen,Yali Xiong,Chen Tian,Bei Jia,Guiyang Wang,Juan Xia,Yuxin Chen,Jian Wang,Xiaomin Yan,Zhaoping Zhang,Rui Huang,Chao Wu

Journal of cellular and molecular medicine 21:3347-3359 PubMed28609022

2017

MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN.

Applications

WB

Species

Unspecified reactive species

Lingqiang Chen,Qin Li,Jing Wang,Song Jin,Hongmei Zheng,Jun Lin,Fang He,Hong Zhang,Sha Ma,Jian Mei,Juan Yu
View all publications
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