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AB53880

Anti-Granulin antibody

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(1 Publication)

Rabbit Polyclonal Granulin antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GRN.

View Alternative Names

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN

1 Images
Western blot - Anti-Granulin antibody (AB53880)
  • WB

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Western blot - Anti-Granulin antibody (AB53880)

Detects bands varying from approximately 64-88 kDa depending on degree of protein glycosylation.

All lanes:

Western blot - Anti-Granulin antibody (ab53880) at 1 µg/mL

Lane 1:

MDA-MB-231 cell lysate

Lane 2:

PC-3 cell lysate

Lane 3:

HeLa cell lysate

Lane 4:

HT1080 cell lysate

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GRN. The exact immunogen used to generate this antibody is proprietary information.

P28799

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the GRN gene is a secreted regulator of lysosomal function and a growth factor involved in processes such as inflammation, wound healing, and cell proliferation. It regulates protein trafficking to lysosomes and lysosomal enzyme activity, facilitating lysosomal acidification which leads to the degradation of mature CTSD by CTSB. It also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation, thus preventing the release of oxidants and proteases. Additionally, it modulates inflammation in neurons by preserving neuron survival, axonal outgrowth, and neuronal integrity. Granulin-3, -4, and -7 have distinct roles : Granulin-4 promotes proliferation of epithelial cell line A431 in culture; Granulin-3 inhibits epithelial cell proliferation and induces IL-8 secretion; and Granulin-7 stabilizes CTSD by maintaining its aspartic-type peptidase activity through interaction. This supplementary information is collated from multiple sources and compiled automatically.
See full target information GRN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:3688 PubMed32703948

2020

Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey Bingor,Tomer Haham,Claire Thornton,Yael Stern-Bach,Rami Yaka
View all publications
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