AB33760

Anti-Granulocytes antibody [HIS48]

Name: Anti-Granulocytes antibody [HIS48] (ab33760) | Abcam
Brand: Abcam Limited
SKU: AB33760
Price: 495 USD
Availability: InStock

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(9 Publications)

Mouse Monoclonal Granulocytes antibody. Suitable for Flow Cyt, IHC-P, ICC/IF, IHC-Fr and reacts with Rat samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing Granulocytes protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIS48

Isotype

IgM

Carrier free

Reacts with

Rat

Applications

Flow Cyt, ICC/IF, IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab3760 recognises granulocytes and erythroid cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 8% Fetal calf serum, 3.16% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulocytes also known as polymorphonuclear leukocytes are immune cells essential for the body's defense mechanism against infections. These cells have a distinct lobed nucleus and are filled with granules that contain enzymes and other biologically active molecules. Weighing between 12-15 kilodaltons granulocytes are expressed in the bone marrow and circulate in the blood. They are classified into three types: neutrophils eosinophils and basophils each with unique granule content and functions.

Biological function summary

Granulocytes play a critical role in the innate immune response by engaging in phagocytosis degranulation and the release of cytokines. Neutrophils are part of the myeloid cell lineage and act as the body's first line of defense by engulfing and destroying pathogens. Eosinophils control parasitic infections and allergic responses while basophils release histamine in allergic reactions. Granulocytes work in coordination with other immune cells such as lymphocytes to form a complex immune system network.

Pathways

Granulocytes participate in pathways that involve inflammatory response and allergic reaction. Neutrophils are integral to the signaling pathway associated with the production of reactive oxygen species which helps eliminate pathogens. This process is linked with proteins like NADPH oxidase. Additionally other pathways involve eosinophils and basophils in modulating immune reactions through cytokine secretion which can also involve mediators like interleukin-5.

Granulocytes have been linked to conditions like chronic granulomatous disease and asthma. Chronic granulomatous disease results from a deficiency in NADPH oxidase impairing the granulocytes' ability to produce reactive oxygen species leading to recurrent infections. Asthma involves an overactive immune response with increased eosinophil activity contributing to airway inflammation. In these contexts granulocytes interact with other proteins including interleukins to modulate the disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Granulocytes

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1363 PubMed39905015

2025

Adoptive cell transfer of piezo-activated macrophage rescues immunosuppressed rodents from life-threating bacterial infections.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Liu,Wenxiu Xu,Junkun Feng,Ying Wang,Kai Li,Yi Chen,Wenjun Wang,Weiwei Zhao,Shaohua Ge,Jianhua Li

Cells 11: PubMed35954280

2022

Add-On Cyclic Angiotensin-(1-7) with Cyclophosphamide Arrests Progressive Kidney Disease in Rats with ANCA Associated Glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Cerullo,Daniela Rottoli,Daniela Corna,Mauro Abbate,Ariela Benigni,Giuseppe Remuzzi,Carlamaria Zoja

Bioactive materials 9:183-197 PubMed34820565

2021

Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Bao,Geng Dou,Ran Tian,Yajie Lv,Feng Ding,Siying Liu,Ruifeng Zhao,Lu Zhao,Jun Zhou,Lin Weng,Yan Dong,Bei Li,Shiyu Liu,Xin Chen,Yan Jin

Scientific reports 11:20684 PubMed34667218

2021

Arginine metabolism and nitric oxide turnover in the ZSF1 animal model for heart failure with preserved ejection fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Petra Büttner,Sarah Werner,Svetlana Baskal,Dimitrios Tsikas,Volker Adams,Philipp Lurz,Christian Besler,Sarah Knauth,Martin Bahls,Edzard Schwedhelm,Holger Thiele

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21655 PubMed34042218

2021

Local shifts in inflammatory and resolving lipid mediators in response to tendon overuse.

Applications

Unspecified application

Species

Unspecified reactive species

James F Markworth,Kristoffer B Sugg,Dylan C Sarver,Krishna Rao Maddipati,Susan V Brooks

Nephron 145:428-444 PubMed33910203

2021

Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Cerullo,Daniela Rottoli,Daniela Corna,Paola Rizzo,Mauro Abbate,Daniela Macconi,Ariela Benigni,Giuseppe Remuzzi,Carlamaria Zoja

JCI insight 5: PubMed32750044

2020

Resolvin D1 supports skeletal myofiber regeneration via actions on myeloid and muscle stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

James F Markworth,Lemuel A Brown,Eunice Lim,Carolyn Floyd,Jacqueline Larouche,Jesus A Castor-Macias,Kristoffer B Sugg,Dylan C Sarver,Peter Cd Macpherson,Carol Davis,Carlos A Aguilar,Krishna Rao Maddipati,Susan V Brooks

Cell 180:984-1001.e22 PubMed32109414

2020

Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ma,Shuhui Sun,Lingling Geng,Moshi Song,Wei Wang,Yanxia Ye,Qianzhao Ji,Zhiran Zou,Si Wang,Xiaojuan He,Wei Li,Concepcion Rodriguez Esteban,Xiao Long,Guoji Guo,Piu Chan,Qi Zhou,Juan Carlos Izpisua Belmonte,Weiqi Zhang,Jing Qu,Guang-Hui Liu

Blood 99:1699-705 PubMed11861286

2002

Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Nagai,Rintaro Shimazu,Hirotaka Ogata,Sachiko Akashi,Katsuko Sudo,Hidetoshi Yamasaki,Shin-Ichi Hayashi,Yoichiro Iwakura,Masao Kimoto,Kensuke Miyake

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Granulocytes antibody [HIS48]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise