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AB75701

Anti-Granulocytes antibody [SPM250]

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(1 Publication)

Mouse Monoclonal Granulocytes antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Granulocytes protein.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granulocytes antibody [SPM250] (AB75701)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granulocytes antibody [SPM250] (AB75701)

ab75701, at a 1/50 dilution, staining Granulocytes in human tonsil tissue by Immunohistochemistry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM250

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab75701 reacts with early precursor and mature forms of human myeloid cells. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "50 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab75701 can be useful for the detection of myeloid leukemias and granulocytic sarcomas and also can be used as a marker of granulocytes in normal tissues or inflammatory processes.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: BSA, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulocytes also known as polymorphonuclear leukocytes are immune cells essential for the body's defense mechanism against infections. These cells have a distinct lobed nucleus and are filled with granules that contain enzymes and other biologically active molecules. Weighing between 12-15 kilodaltons granulocytes are expressed in the bone marrow and circulate in the blood. They are classified into three types: neutrophils eosinophils and basophils each with unique granule content and functions.
Biological function summary

Granulocytes play a critical role in the innate immune response by engaging in phagocytosis degranulation and the release of cytokines. Neutrophils are part of the myeloid cell lineage and act as the body's first line of defense by engulfing and destroying pathogens. Eosinophils control parasitic infections and allergic responses while basophils release histamine in allergic reactions. Granulocytes work in coordination with other immune cells such as lymphocytes to form a complex immune system network.

Pathways

Granulocytes participate in pathways that involve inflammatory response and allergic reaction. Neutrophils are integral to the signaling pathway associated with the production of reactive oxygen species which helps eliminate pathogens. This process is linked with proteins like NADPH oxidase. Additionally other pathways involve eosinophils and basophils in modulating immune reactions through cytokine secretion which can also involve mediators like interleukin-5.

Granulocytes have been linked to conditions like chronic granulomatous disease and asthma. Chronic granulomatous disease results from a deficiency in NADPH oxidase impairing the granulocytes' ability to produce reactive oxygen species leading to recurrent infections. Asthma involves an overactive immune response with increased eosinophil activity contributing to airway inflammation. In these contexts granulocytes interact with other proteins including interleukins to modulate the disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Granulocytes
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology 50:467-79 PubMed25112701

2014

Overexpression and gene amplification of EGFR, HER2, and HER3 in biliary tract carcinomas, and the possibility for therapy with the HER2-targeting antibody pertuzumab.

Applications

Unspecified application

Species

Unspecified reactive species

Toru Kawamoto,Kazunori Ishige,Melanie Thomas,Yoriko Yamashita-Kashima,Sei Shu,Nobuyuki Ishikura,Shunichi Ariizumi,Masakazu Yamamoto,Kunihiko Kurosaki,Junichi Shoda
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Product promise

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