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AB224047

Anti-Granuphilin antibody

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(2 Publications)

Rabbit Polyclonal Granuphilin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SYTL4 aa 150-350.

View Alternative Names

Synaptotagmin-like protein 4, Exophilin-2, Granuphilin, SYTL4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Granuphilin antibody (AB224047)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Granuphilin antibody (AB224047)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Granuphilin (green) using ab224047 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB224047)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB224047)

Paraffin-embedded human pancreas tissue stained for Granuphilin using ab224047 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-Granuphilin antibody (AB224047)
  • WB

Supplier Data

Western blot - Anti-Granuphilin antibody (AB224047)

All lanes:

Western blot - Anti-Granuphilin antibody (ab224047) at 1/250 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate (negative control)

Lane 2:

Granuphilin over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SYTL4 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q96C24

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granuphilin also known as synaptotagmin-like protein 4 (SYTL4) serves as an important player in vesicle trafficking and fusion with its role as a Rab effector protein. It weighs approximately 85 kDa. This protein mainly expresses in insulin-secreting beta cells of the pancreas and in adipocytes. Granuphilin directly binds to Rab3 and Rab27 influencing the movement and docking of secretory granules on the plasma membrane.
Biological function summary

Granuphilin impacts insulin secretion by managing the docking of insulin granules. It is an essential regulator within the secretory pathway. Granuphilin does not work alone but instead associates with a complex of proteins including Rab27A to facilitate precise targeting and fusion of vesicles with cell membranes. This interaction impacts the exocytosis process vital for hormone release.

Pathways

Granuphilin integrates into the insulin secretion pathway and the exocytosis pathway. It acts alongside proteins such as Rab3 influencing the regulation of granule docking and secretion from pancreatic beta cells. By regulating these pathways granuphilin plays a role in glucose homeostasis and helps maintain metabolic balance within the body.

Granuphilin shows a connection to type 2 diabetes and obesity. Disruption in granuphilin functions can lead to impaired insulin secretion contributing the development to type 2 diabetes. Additionally the protein's involvement in adipocyte-related pathways suggests a role in obesity-related mechanisms. Granuphilin interacts with Rab27 in these contexts linking its malfunction to these metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner (By similarity).
See full target information SYTL4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:877 PubMed34564708

2021

Cardiac Wnt5a and Wnt11 promote fibrosis by the crosstalk of FZD5 and EGFR signaling under pressure overload.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zou,Le Pan,Yi Shen,Xiang Wang,Chenxing Huang,Hao Wang,Xuejuan Jin,Chao Yin,Ying Wang,Jianguo Jia,Juying Qian,Yunzeng Zou,Hui Gong,Junbo Ge

Nature communications 12:3970 PubMed34172739

2021

The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bijeta Prasai,Gideon J Haber,Marie-Paule Strub,Regina Ahn,John A Ciemniecki,Kem A Sochacki,Justin W Taraska
View all publications
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Product promise

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