Rabbit Polyclonal Granuphilin antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SYTL4.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SYTL4. The exact immunogen used to generate this antibody is proprietary information. Database link Q96C24
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner (By similarity).
Alternative names
Synaptotagmin-like protein 4, Exophilin-2, Granuphilin, SYTL4
Recommended products
Rabbit Polyclonal Granuphilin antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SYTL4.
Alternative names
Synaptotagmin-like protein 4, Exophilin-2, Granuphilin, SYTL4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SYTL4. The exact immunogen used to generate this antibody is proprietary information. Database link Q96C24
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Granuphilin also known as synaptotagmin-like protein 4 (SYTL4) serves as an important player in vesicle trafficking and fusion with its role as a Rab effector protein. It weighs approximately 85 kDa. This protein mainly expresses in insulin-secreting beta cells of the pancreas and in adipocytes. Granuphilin directly binds to Rab3 and Rab27 influencing the movement and docking of secretory granules on the plasma membrane.
- Biological function summary
Granuphilin impacts insulin secretion by managing the docking of insulin granules. It is an essential regulator within the secretory pathway. Granuphilin does not work alone but instead associates with a complex of proteins including Rab27A to facilitate precise targeting and fusion of vesicles with cell membranes. This interaction impacts the exocytosis process vital for hormone release.
- Pathways
Granuphilin integrates into the insulin secretion pathway and the exocytosis pathway. It acts alongside proteins such as Rab3 influencing the regulation of granule docking and secretion from pancreatic beta cells. By regulating these pathways granuphilin plays a role in glucose homeostasis and helps maintain metabolic balance within the body.
- Associated diseases and disorders
Granuphilin shows a connection to type 2 diabetes and obesity. Disruption in granuphilin functions can lead to impaired insulin secretion contributing the development to type 2 diabetes. Additionally the protein's involvement in adipocyte-related pathways suggests a role in obesity-related mechanisms. Granuphilin interacts with Rab27 in these contexts linking its malfunction to these metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (ab228892)
Paraffin-embedded mouse ovary tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Granuphilin antibody (ab228892)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Granuphilin antibody (ab228892) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extracts at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (ab228892)
Paraffin-embedded mouse pancreas tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.
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