JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB228892

Anti-Granuphilin antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Granuphilin antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SYTL4.

View Alternative Names

Synaptotagmin-like protein 4, Exophilin-2, Granuphilin, SYTL4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB228892)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB228892)

Paraffin-embedded mouse ovary tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB228892)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB228892)

Paraffin-embedded mouse pancreas tissue stained for Granuphilin using ab228892 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Granuphilin antibody (AB228892)
  • WB

Supplier Data

Western blot - Anti-Granuphilin antibody (AB228892)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Granuphilin antibody (ab228892) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell extracts at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg

Predicted band size: 76 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SYTL4. The exact immunogen used to generate this antibody is proprietary information.

Q96C24

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granuphilin also known as synaptotagmin-like protein 4 (SYTL4) serves as an important player in vesicle trafficking and fusion with its role as a Rab effector protein. It weighs approximately 85 kDa. This protein mainly expresses in insulin-secreting beta cells of the pancreas and in adipocytes. Granuphilin directly binds to Rab3 and Rab27 influencing the movement and docking of secretory granules on the plasma membrane.
Biological function summary

Granuphilin impacts insulin secretion by managing the docking of insulin granules. It is an essential regulator within the secretory pathway. Granuphilin does not work alone but instead associates with a complex of proteins including Rab27A to facilitate precise targeting and fusion of vesicles with cell membranes. This interaction impacts the exocytosis process vital for hormone release.

Pathways

Granuphilin integrates into the insulin secretion pathway and the exocytosis pathway. It acts alongside proteins such as Rab3 influencing the regulation of granule docking and secretion from pancreatic beta cells. By regulating these pathways granuphilin plays a role in glucose homeostasis and helps maintain metabolic balance within the body.

Granuphilin shows a connection to type 2 diabetes and obesity. Disruption in granuphilin functions can lead to impaired insulin secretion contributing the development to type 2 diabetes. Additionally the protein's involvement in adipocyte-related pathways suggests a role in obesity-related mechanisms. Granuphilin interacts with Rab27 in these contexts linking its malfunction to these metabolic disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner (By similarity).
See full target information SYTL4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:1062695 PubMed36568562

2022

Large-conductance Ca-activated K channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meili Wang,Shuanglei Li,Hongshan Liu,Mingyuan Liu,Jin Zhang,Yang Wu,Cangsong Xiao,Haixia Huang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB224047

Anti-Granuphilin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granuphilin antibody (AB224047)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB228833

Anti-ZNF556 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZNF556 antibody (AB228833)

  • 0
  • 0
Primary Antibodies

AB228574

Anti-ZNF45 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF45 antibody (AB228574)

  • 0
  • 0
Primary Antibodies

AB3338

Anti-Rabphilin 3A antibody

Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (AB3338)

  • 4
  • 1
Primary Antibodies

AB228680

Anti-ThPok antibody

Western blot - Anti-ThPok antibody (AB228680)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com