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AB10870

Anti-Granzyme A antibody [GA6]

4

(1 Review)

|

(4 Publications)

Mouse Monoclonal Granzyme A antibody. Suitable for WB, IHC-FoFr, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GZMA.

View Alternative Names

CTLA3, HFSP, GZMA, Granzyme A, CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GA6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-FoFr, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GZMA.

P12544

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Alternative Products

Primary Antibodies

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Complementary Products

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

AB282703

Anti-Granzyme K antibody [EPR24601-164]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Recognises a 60kD disulphide-linked homodimeric protein.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified IgG prepared by affinity chromatography from tissue culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme A also known as CTLA3 or GZMA is a serine protease with a role in immune cell-mediated cytotoxicity. It has a molecular weight of approximately 27 kDa. This protein is primarily expressed in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Granzyme A gets released into the immunological synapse upon recognition of target cells by these immune cells facilitating the induction of target cell death.
Biological function summary

Functions of Granzyme A extend to inducing apoptosis in target cells alongside its role in promoting inflammation. It operates independent of caspases and directly cleaves substrates in the cell to induce cell death making it different from the related Granzyme B. As part of the granzyme-perforin complex it enters target cells via perforin-created pores mediates reactions leading to substrate degradation and promotes DNA damage.

Pathways

Granzyme A gets involved in the granule exocytosis pathway which constitutes an important mechanism for CTLs and NK cells to kill infected or cancerous cells. The protein is also linked to the inflammatory response pathways where it can interact with other serine proteases. Related proteins like perforin and Granzyme B also engage in these pathways and they collectively work to trigger apoptosis and modulate immune responses.

Impairments or dysregulation of Granzyme A function can contribute to autoimmune diseases and certain viral infections. Autoimmune diseases such as lupus may demonstrate altered levels or activity of Granzyme A associated with tissue damage and inflammation. During viral infections Granzyme A in conjunction with perforin and Granzyme B aids in eliminating infected cells but alterations in this balance could lead to ineffective immune responses or excessive tissue damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 12819770, PubMed : 32299851, PubMed : 3257574, PubMed : 3262682, PubMed : 3263427). It cleaves after Lys or Arg (PubMed : 12819770, PubMed : 32299851). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death (PubMed : 32299851, PubMed : 34022140, PubMed : 36157507, PubMed : 36899106). Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity (PubMed : 12524539). Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (PubMed : 11555662, PubMed : 12628186, PubMed : 16818237).
See full target information GZMA
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancers 12: PubMed32645996

2020

Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPα Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Papalampros,Michail Vailas,Konstantinos Ntostoglou,Maria Lopez Chiloeches,Stratigoula Sakellariou,Niki V Chouliari,Menelaos G Samaras,Paraskevi D Veltsista,Sofia D P Theodorou,Aggelos T Margetis,Anna Bergonzini,Lysandros Karydakis,Natasha Hasemaki,Sophia Havaki,Ioannis I Moustakas,Antonios Chatzigeorgiou,Timokratis Karamitros,Eleni Patsea,Christos Kittas,Andreas C Lazaris,Evangelos Felekouras,Vassilis G Gorgoulis,Teresa Frisan,Ioannis S Pateras

The Journal of pathology 251:272-283 PubMed32418210

2020

Molecular subtyping reveals immune alterations in IDH wild-type lower-grade diffuse glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wu,Guan-Zhang Li,Han-Jie Liu,Zheng Zhao,Rui-Chao Chai,Yu-Qing Liu,Hao-Yu Jiang,You Zhai,Yue-Mei Feng,Ren-Peng Li,Wei Zhang

PloS one 7:e37030 PubMed22719835

2012

Glucocorticoid receptor and sequential P53 activation by dexamethasone mediates apoptosis and cell cycle arrest of osteoblastic MC3T3-E1 cells.

Applications

WB

Species

Mouse

Hui Li,Wenwei Qian,Xisheng Weng,Zhihong Wu,Huihua Li,Qianyu Zhuang,Bin Feng,Yanyan Bian

Cancer science 101:259-66 PubMed19817750

2009

Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling.

Applications

IHC-FoFr

Species

Human

Seiko Hirono,Hiroki Yamaue,Yutaka Hoshikawa,Shinomi Ina,Masaji Tani,Manabu Kawai,Masaru Ushijima,Masaaki Matsuura,Yuriko Saiki,Akio Saiura,Junji Yamamoto,Yoshio Miki,Tetsuo Noda
View all publications
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Product promise

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