Rabbit Polyclonal Granzyme B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 165 publications.
Images
Publications
Filter by
- Nature biomedical engineering 8:345-3602023Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarius F Harter,Timothy Recaldin,Regine Gerard,Blandine Avignon,Yannik Bollen,Cinzia Esposito,Karolina Guja-Jarosz,Kristina Kromer,Adrian Filip,Julien Aubert,Anneliese Schneider,Marina Bacac,Michael Bscheider,Nadine Stokar-Regenscheit,Salvatore Piscuoglio,Joep Beumer,Nikolche GjorevskiPubMed 38114742
- Nature communications 14:72352023Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiren Wang,Wenpan Li,Yanhao Jiang,Tuyen Ba Tran,Leyla Estrella Cordova,Jinha Chung,Minhyeok Kim,Georg Wondrak,Jennifer Erdrich,Jianqin LuPubMed 37945606
- iScience 26:1083532023TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyle B Lupo,Sandra Torregrosa-Allen,Bennett D Elzey,Sagar Utturkar,Nadia A Lanman,Aaron A Cohen-Gadol,Veronika Slivova,MacKenzie McIntosh,Karen E Pollok,Sandro MatosevicPubMed 38053639
- Nature communications 14:56652023MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZinab O Doha,Xiaoyan Wang,Nicholas L Calistri,Jennifer Eng,Colin J Daniel,Luke Ternes,Eun Na Kim,Carl Pelz,Michael Munks,Courtney Betts,Sunjong Kwon,Elmar Bucher,Xi Li,Trent Waugh,Zuzana Tatarova,Dylan Blumberg,Aaron Ko,Nell Kirchberger,Jennifer A Pietenpol,Melinda E Sanders,Ellen M Langer,Mu-Shui Dai,Gordon Mills,Koei Chin,Young Hwan Chang,Lisa M Coussens,Joe W Gray,Laura M Heiser,Rosalie C SearsPubMed 37704631
- Biomedicines 11:2023Combined Inhibition of Indolamine-2,3-Dioxygenase 1 and C-X-C Chemokine Receptor Type 2 Exerts Antitumor Effects in a Preclinical Model of Cervical Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSolangy Lizcano-Meneses,Rogelio Hernández-Pando,Ian García-Aguirre,José Bonilla-Delgado,Víctor Manuel Alvarado-Castro,Bulmaro Cisneros,Patricio Gariglio,Enoc Mariano Cortés-MalagónPubMed 37626777
- Journal of experimental & clinical cancer research : CR 42:1922023Lysosome blockade induces divergent metabolic programs in macrophages and tumours for cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Ma,Ruijuan Ma,Xueke Zeng,Liming Zhang,Jianing Liu,Wei Zhang,Tao Li,Hanjing Niu,Guochen Bao,Chaojie Wang,Peng George Wang,Jiajia Wang,Xia Li,Taotao Zou,Songqiang XiePubMed 37537587
- Cancer immunology, immunotherapy : CII 72:3593-36082023Reovirus combined with a STING agonist enhances anti-tumor immunity in a mouse model of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaomi Sugimura,Eiji Kubota,Yoshinori Mori,Mineyoshi Aoyama,Mamoru Tanaka,Takaya Shimura,Satoshi Tanida,Randal N Johnston,Hiromi KataokaPubMed 37526659
- Frontiers in cellular and infection microbiology 13:11657902023Intratumoral microbiome impacts immune infiltrates in tumor microenvironment and predicts prognosis in esophageal squamous cell carcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuyue Zhang,Shuishen Zhang,Xiaofan Ma,Jing Zhan,Chuqing Pan,Huizhong Zhang,Xiuying Xie,Jing Wen,Xuan XiePubMed 37180444
- Journal of cellular physiology 238:1507-15192023IFI16/Ifi202 released from breast cancer induces secretion of inflammatory cytokines from macrophages and promotes tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGa Young Lim,Sun Wook Cho,Na-Lee Ka,Kyung-Hun Lee,Seock-Ah Im,Seung-Su Kim,Sewon Hwang,Mi-Ock LeePubMed 37062941
- MedComm 4:e2422023Macrophages facilitate tumor cell PD-L1 expression via an IL-1β-centered loop to attenuate immune checkpoint blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Xu,Yu Xia,Bai-Wei Zhang,Emmanuel Kwateng Drokow,Hua-Yi Li,Sen Xu,Zhen Wang,Si-Yuan Wang,Ping Jin,Tian Fang,Xiao-Ming Xiong,Pu Huang,Ning Jin,Jia-Hong Tan,Qing Zhong,Yu-Xin Chen,Qi Zhang,Yong Fang,Fei Ye,Qing-Lei GaoPubMed 37009412
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Common marmoset | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Common marmoset | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927). It cleaves after Asp (PubMed:8258716, PubMed:1985927). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).
Alternative names
Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP, GRB, CTLA1, CSPB, CGL1, GZMB
Recommended products
Rabbit Polyclonal Granzyme B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 165 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Huszthy et al PLoS One. 2015 Aug 20;10(8):e0136089. doi: 10.1371/journal.pone.0136089. eCollection 2015. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)
Cytotoxic effector cells and regulatory T cells in human glioblastoma xenograft growing in immunocompetent rats.
Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)
Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com