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AB53097

Anti-Granzyme B antibody

4

(5 Reviews)

|

(12 Publications)

Rabbit Polyclonal Granzyme B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human GZMB.

View Alternative Names

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB53097)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB53097)

ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.

Western blot - Anti-Granzyme B antibody (AB53097)
  • WB

Unknown

Western blot - Anti-Granzyme B antibody (AB53097)

All lanes:

Western blot - Anti-Granzyme B antibody (ab53097) at 1/300 dilution

Lane 1:

NIH/3T3 cell extract

Lane 2:

NIH/3T3 cell extract with immunizing peptide

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GZMB. The exact immunogen used to generate this antibody is proprietary information.

P10144

Specificity

Detects endogenous levels of total Granzyme B protein. Due to sequence homology this antibody is predicted to cross-react with granzyme H.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "50-100 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
See full target information GZMB
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 32:3597-3617 PubMed39066478

2024

Specific targeting of cancer vaccines to antigen-presenting cells via an endogenous TLR2/6 ligand derived from cysteinyl-tRNA synthetase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeong Yun Kim,Seongmin Cho,Sang Bum Kim,Ee Chan Song,Wonchul Jung,Yun Gyeong Shin,Ji Hun Suh,Jihye Choi,Ina Yoon,Uijoo Kim,Hamin Ban,Sunkyo Hwang,Jeongwon Mun,Joohee Park,Nayoung Kim,Youngjin Lee,Myung Hee Kim,Sunghoon Kim

The Journal of allergy and clinical immunology 152:1312-1320.e3 PubMed37536509

2023

Monensin induces secretory granule-mediated cell death in eosinophils.

Applications

Unspecified application

Species

Unspecified reactive species

Marianthi Vraila,Elin Asp,Fabio Rabelo Melo,Mirjana Grujic,Ola Rollman,Gunnar Pejler,Maria Lampinen

BioMed research international 2022:1557010 PubMed35677097

2022

Therapeutical Significance of Serpina3n Subsequent Cerebral Ischemia via Cytotoxic Granzyme B Inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Mehwish Saba Aslam,Mobeena Saba Aslam,Komal Saba Aslam,Asia Iqbal,Liudi Yuan

iScience 25:103785 PubMed35146396

2022

PD-L1CD8 T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxia Zheng,Li Han,Zheyi Chen,Yiyang Li,Bingqian Zhou,Rui Hu,Shiyu Chen,Haibo Xiao,Yanhui Ma,Guohua Xie,Junyao Yang,Xianting Ding,Lisong Shen

Molecules (Basel, Switzerland) 26: PubMed34946538

2021

Attenuation of Tumor Development in Mammary Carcinoma Rats by Theacrine, an Antagonist of Adenosine 2A Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Cian-Fen Jhuo,Yu-Yu Hsu,Wen-Ying Chen,Jason T C Tzen

The Journal of pharmacology and experimental therapeutics 368:401-413 PubMed30591531

2018

Inhibition of COX-2 and EGFR by Melafolone Improves Anti-PD-1 Therapy through Vascular Normalization and PD-L1 Downregulation in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Honglin Tang,Yanzhuo Liu,Chenlong Wang,Hao Zheng,Yaxin Chen,Wen Liu,Xuewei Chen,Jing Zhang,Honglei Chen,Yuqing Yang,Jing Yang

Turkish neurosurgery 28:571-581 PubMed30192361

2018

Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin.

Applications

Unspecified application

Species

Unspecified reactive species

Alican Tahta,Nail Izgi,Tugba Bagci-Onder,Ece Erdag,Yavuz Aras,Cetin Genc

Molecular medicine reports 17:8530-8535 PubMed29658580

2018

Norepinephrine inhibits the cytotoxicity of NK92‑MI cells via the β2‑adrenoceptor/cAMP/PKA/p‑CREB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zan Sun,Diandong Hou,Shuli Liu,Weixin Fu,Jiahui Wang,Zaifu Liang

Experimental and therapeutic medicine 12:3773-3777 PubMed28105109

2016

Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Sun,Mofei Guo,Yuanjie Feng,Huifang Zheng,Ping Lei,Xiande Ma,Xiaowei Han,Hongquan Guan,Diandong Hou

Molecular cancer therapeutics 12:2055-66 PubMed23858102

2013

The functionalized human serine protease granzyme B/VEGF₁₂₁ targets tumor vasculature and ablates tumor growth.

Applications

ICC/IF

Species

Unspecified reactive species

Khalid A Mohamedali,Yu Cao,Lawrence H Cheung,Walter N Hittelman,Michael G Rosenblum
View all publications
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