Anti-Granzyme B antibody [BLR022E]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling Granzyme B with ab243879.

Positive staining on human lymph node

Counterstained with Hematoxylin.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B (shaded) in SR (human lymphoma cell line) cells by flow cytometry.

SR cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10⁶ cells.

Antibody : Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (ab243879) or isotype control (unshaded).

Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (ab96886).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Granzyme B with ab243879.

Positive staining on human lung carcinoma

Counterstained with Hematoxylin.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Formalin-fixed, paraffin-embedded human lung carcinoma stained for Granzyme B (red) using ab243879 at 1/250 dilution in immunohistochemical analysis. HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B by immunocytochemistry.

Sample : Cell pellet FFPE section of Granzyme B over-expressing cells (left) and Granzyme H over-expressing cells (right).

Antibody : Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (ab243879).

Secondary : HRP-conjugated goat anti-rabbit IgG.

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

• WB

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Western blot - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B by western blot.

In Western blot, Rabbit anti-COPB2 antibody (ab192924) - used as a Loading Control, lower panel.

Samples prepared using NETN lysis buffer.

Chemiluminescence detection.

All lanes:

Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879) at 1/1000 dilution

Lane 1:

HEK293T (Human Embryonic Kidney cells) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

SR (human lymphoblast cell line) whole cell lysate at 15 µg

Lane 4:

Jurkat (human T-lymphoblastic leukemia cell line) whole cell lysate at 15 µg

Lane 5:

Hep-G2 (Human Hepatoblastoma Cell Line) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 27.7 kDa

Observed band size: 38 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B by western blot.

In Western blot, Rabbit anti-myc antibody (ab314108) lower panel.

Samples Myc-tagged Granzyme H and B overexpressing cell lysates prepared using NETN lysis buffer.

Chemiluminescence detection.

All lanes:

Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879) at 1/1000 dilution

Lane 1:

Myc-tagged Granzyme H overexpressing cell lysate at 15 µg

Lane 2:

Myc-tagged Granzyme B overexpressing cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 27.7 kDa

Observed band size: 38 kDa

false

Exposure time: 3s